Abstract
The ease of use, robustness, cost-effectiveness, and posttranslational machinery make baculovirus expression system a popular choice for production of eukaryotic membrane proteins. This system can be readily adapted for high-throughput operations. This chapter outlines the techniques and procedures for cloning, transfection, small-scale production, and purification of membrane protein samples in a high-throughput manner.
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This work was supported by NIH grant U54 GM095315-04.
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Kalathur, R.C., Panganiban, M., Bruni, R. (2016). High-Throughput Baculovirus Expression System for Membrane Protein Production. In: Mus-Veteau, I. (eds) Heterologous Expression of Membrane Proteins. Methods in Molecular Biology, vol 1432. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3637-3_12
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DOI: https://doi.org/10.1007/978-1-4939-3637-3_12
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