Abstract
The baculovirus expression vector system (BEVS) is one of the most popular eukaryotic systems for recombinant protein production. The focus of our protein production platform is the provision of recombinant proteins for research use, where generally only small quantities are required, in the range of tens of micrograms to a few hundred milligrams. Here, we present methods that reflect our standard operating procedures and setup to be able to frequently, and often repeatedly, produce many different types of proteins.
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Acknowledgments and Funding
We thank Lund University for its financial support of the Lund Protein Production Platform (LP3). Protein Production Sweden (PPS) is funded by the Swedish Research Council as a national research infrastructure.
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Sullivan, H.M., Krupinska, E., Rasmussen, A.A., Orozco Rodriguez, J.M., Knecht, W. (2023). Recombinant Protein Production Using the Baculovirus Expression Vector System (BEVS). In: Sousa, Â., Passarinha, L. (eds) Advanced Methods in Structural Biology. Methods in Molecular Biology, vol 2652. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3147-8_4
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DOI: https://doi.org/10.1007/978-1-0716-3147-8_4
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