Abstract
Radiation treatment of genomes is used to generate chromosome breaks for numerous applications. This protocol describes the preparation of seeds and the determination of the optimal level of irradiation dosage for the creation of a radiation hybrid (RH) population. These RH lines can be used to generate high-resolution physical maps for the assembly of sequenced genomes as well as the fine mapping of genes. This procedure can also be used for mutation breeding and forward/reverse genetics.
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Acknowledgments
Funding from the National Science Foundation, Plant Genome Research Program (NSF-PGRP) grant No. IOS-0822100 to SFK is gratefully acknowledged. Numerous students and postdoctoral scientists participate in the work on radiation treatments of wheat, barley, maize and sorghum at North Dakota State University to list here but are named among the many publications referenced in this article.
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Kianian, P.M.A., Liberatore, K.L., Miller, M.E., Hegstad, J.B., Kianian, S.F. (2016). Dissecting Plant Chromosomes by the Use of Ionizing Radiation. In: Kianian, S., Kianian, P. (eds) Plant Cytogenetics. Methods in Molecular Biology, vol 1429. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3622-9_8
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DOI: https://doi.org/10.1007/978-1-4939-3622-9_8
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Online ISBN: 978-1-4939-3622-9
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