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On the optimum mutation rate and optimum dose for practical mutation breeding

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Optimum mutation rates for practical purpose were discussed based on the theoretically derived formulae, and mutagen doses giving the optimum mutation rates were estimated from the experiments reported hitherto. It was suggested that in cereal crops such as rice and barley, considerably low mutation rates as with the mutagen doses lower than 5 kR of λ or X rays and 2%·h of EMS are appropriate for obtaining directly applicable mutants. High mutation rates seem to be limited to the production of some rare breeding stocks.

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References

  • Ando, A., 1970. Mutation induction in rice by radiation combined with chemical protectants and mutagens. In: Rice breeding with induced mutations. pp. 7–15. IAEA, Vienna.

    Google Scholar 

  • Arnason, T. J., L. Mohammed, D. Koehler & F. M. Rennerberg, 1962. Mutation frequencies in barley after treatment with λ-radiation, ethylene imine, ethyl methanesulfonate and maleic hydrazide. Can. J. Genet. Cytol. 4: 172–178.

    Google Scholar 

  • Beermann, W., 1967. Gene action at the level of the chromosome. In: R. A. Brink and E. D. Styles (Eds). Heritage from Mendel. pp. 179–201 The University of Wisconsin Press, Madison, Wisconsin.

    Google Scholar 

  • Bender, K. & H. Gaul, 1966. Nachwäsche, Rücktrocknung und Lagerung bei ÄMS-behandelten Gerstensamen. Radiation Botany 6: 505–518.

    Google Scholar 

  • Borojevic, K., 1970. Differences depending on the genotype. In: Manual on mutation breeding. pp. 125–126. IAEA, Vienna.

    Google Scholar 

  • Braidwood, R. J., 1970. The agricultural revolution. In: Plant agriculture. pp. 4–12. W. H. Freeman and Company, San Francisco.

    Google Scholar 

  • Brock, R. D. 1969. Increasing the specificity of mutation. In: Induced mutations in plants. pp. 93–100 IAEA. Vienna.

    Google Scholar 

  • Diamantis, B., 1974. A mathematical investigation of the induced mutation rate which is optimum for genetic improvement. Part I. Mutagenic treatment of the haploid: the three-locus case. Theor. appl. Genetics 44: 31–44.

    Google Scholar 

  • Djalali, M., W. Hoffmann & W. Plarre, 1970. Genetics and variability of the labile-gene in barley under different environmental conditions. In: Barley genetics II. pp. 201–207. Washington State University Press. Washington.

    Google Scholar 

  • Doll, H. & J. Sandfaer, 1969. Mutagenic effect of gamma rays, diethyl sulphate, ethyl methane-sulphonate, and various combinations of gamma rays and the chemicals. In: Induced mutations in plants. pp. 195–206. IAEA, Vienna.

    Google Scholar 

  • Ehrenberg, L., U. Lundqvist, S. Osterman & B. Sparrman, 1966. On the mutagenic action of alkanesulfonic esters in barley. Hereditas 56: 277–305.

    Google Scholar 

  • Eriksson, G., 1965. The size of the mutated sector in barley spikes estimated by means of waxy mutants. Hereditas 53: 307–326.

    Google Scholar 

  • Favret, E. A., 1964. Genetic effects of single and combined treatment of ionizing radiations and ethyl methanesulphonate on barley seeds. In: Barley genetics I. pp. 68–81. Proc. 1st Intern. Barley Genetics Symp. Wageningen, 1963. Pudoc. Wageningen.

    Google Scholar 

  • Froese-Gertzen, E. E. & C. Konzak, 1964. The effects of ethyl methanesulphonate on the growth response, chromosome structure and mutation rate in barley. Radiation Botany 4: 61–69.

    Google Scholar 

  • Fuke, Y., 1954. Genetical study of the photoperiodic reaction in the rice plant. Bull. natn. Inst. agric. Sci., Ser. D, 5: 1–91.

    Google Scholar 

  • Futsuhara, Y., 1963. Selection methods of mutants. In: Recent advance in breeding science. pp. 88–95. Yokendo, Tokyo.

    Google Scholar 

  • Futsuhara, Y., K. Toriyama & K. Tsunoda, 1967. Breeding of a new rice variety ‘Reimei’ by gamma-ray irradiation. Japan. J. Breed. 17: 85–90.

    Google Scholar 

  • Gaul, H., 1961. Studies on diplontic selection after X-irradiation of barley seeds. In: Effects of ionizing radiations on seeds. pp. 117–138. IAEA, Vienna.

    Google Scholar 

  • Gaul, H., 1964. Mutations in plant breeding. Radiation Botany 4: 155–232.

    Google Scholar 

  • Gaul, H., J. Grünewaldt & C. U. Hesemann, 1968. Variation of character expression of barley mutants in a changed genetic background. In: Mutations in plant breeding II. pp. 77–96. IAEA, Vienna.

    Google Scholar 

  • Gaul, H., E. Ulonska, C. zum Winkel & G. Braker, 1969. Micro-mutations influencing yield in barley-studies over nine generations. In: Induced mutations in plants. pp. 375–398. IAEA, Vienna.

    Google Scholar 

  • Gottschalk, W., 1968. Simultaneous mutation of closely linked genes. A contribution to the interpretation of ‘pleiotropic’ gene action. In: Mutations in plant breeding II. pp. 97–109. IAEA, Vienna.

    Google Scholar 

  • Gustaffson, Å., 1956. Studies on the experimental control of the mutation process. In: Proc. Radiobiol. Symp. pp. 282–284, Liege, Academic Press., New York.

    Google Scholar 

  • Hänsel, H., 1965. Untersuchungen über die Häufigkeit induzierter chlorophyllmutationen nach ÄMS-Behandlung, Neutronen-und Röntogenbestrahlung von Gerstensamen. Die Bodenkultur 16: 325–339.

    Google Scholar 

  • Hänsel, H., 1967. Model for a theoretical estimate of optimal mutation rates per M1-nucleus with a view to selecting beneficial mutations in different M-generation. In: Induced mutations and their utilization. pp. 79–87. Akademic-Verlag, Berlin.

    Google Scholar 

  • Hänsel, H., 1968. An estimate of the rate of chlorophyll mutations in barley taking account of multiply mutated M1-nuclei. In: Mutation in plant breeding II. pp. 139–151. IAEA, Vienna.

    Google Scholar 

  • Kawal, T., 1963. Comparison between the mutagenic effectiveness of outer and inner irradiations. In: Recent advance in breeding science. pp. 70–78. Yokendo, Tokyo.

    Google Scholar 

  • Kihara, H., T. Morinaga, Y. Sinoda, H. Tsukuba, T. Uchida & M. Ueno, 1973. The history of biological sciences in relation to cultivated plants and domesticated animals in Old Japan. p. 436. Yokendo. Tokyo.

    Google Scholar 

  • King, J. L. & T. H. Jukes, 1969. Non-Darwinian evolution. Science 164: 788–798.

    Google Scholar 

  • Konzak, C. F., R. A. Nilan, J. R. Harle & E. E. Heiner, 1961. Control of factors affecting the response of plants to mutagens. Brookhaven Symp. Biol. 14: 128–157.

    Google Scholar 

  • Konzak, C. F., R. A. Nilan, J. Wagner & R. J. Foster, 1965. Efficient chemical mutagenesis. In: The use of induced mutations in plant breeding. pp. 49–70, IAEA, Vienna.

    Google Scholar 

  • Lundqvist, U. & D. von Wettstein, 1962. Induction of eceriferum mutants in barley by ionizing radiations and chemical mutagens. Hereditas 48: 342–362.

    Google Scholar 

  • Matsuo, T. & Y. Onozawa, 1960. Studies on mutations induced by radiations and chemicals. 1. The effect of combination of radiation and chemicals on the growth and mutation frequency of rice. Japan. J. Breed. 10: 223–227.

    Google Scholar 

  • Matsuo, T., H. Yamaguchi & A. Ando, 1958. A comparison of biological effects between thermal neutron and X-rays on rice seeds. Japan. J. Breed. 8: 37–45.

    Google Scholar 

  • Mikaelsen, K., 1969. Influence of mitotic stage on the effectiveness of mutagen treatments. In: Induced mutations in plants. pp. 245–249, IAEA, Vienna.

    Google Scholar 

  • Mitra, R. & C. R. Bhatia, 1973. Repeated and non-repeated nucleotide sequences in diploid and polyploid wheat species. Heredity 31: 251–262.

    Google Scholar 

  • Mose, A., 1964. Comparison of the effects of X-rays and of ethyl methanesulphonate in barley. In: Barley genetics I. pp. 82–91. Proc. 1st Intern. Barley Genetics Symp., Wageningen, 1963. Pudoc, Wageningen.

    Google Scholar 

  • Nilan, R. A., 1966. Nature of induced mutations in higher plants. In: Induced mutations and their utilization. pp. 5–20. Akademie-Verlag, Berlin.

    Google Scholar 

  • Nilan, R. A., C. F. Konzak, R. E. Heiner & E. E. Froese-Gertzen, 1964. Chemical mutagenesis in barley. In: Barley genetics I. pp. 35–54. Proc. 1st Intern. Barely Genetics Symp., Wageningen, 1963. Pudoc, Wageningen.

    Google Scholar 

  • Oka, H., 1953. Phylogenetic differentiation of the cultivated rice plant. I. Variation of various characters and character combinations among rice varieties. Japan. J. Breed. 3: 33–64.

    Google Scholar 

  • Osone, K., 1966. Comparison of mutagenic effects of ethyleneimine and ionizing radiations on rice. Gamma Field Symp. 5: 53–59.

    Google Scholar 

  • Rahman, M. M. & J. D. Soriano, 1972. Studies on the mutagenic effects of some monofunctional alkylating agents in rice. Radiation Botany 12: 291–295.

    Google Scholar 

  • Robertson, A., 1967. The nature of quantitative genetic variation. In: R. A. Brink & E. D. Styles (Eds). Heritage from Mendel. pp. 265–280. The University of Wisconsin Press, Madison, Wisconsin.

    Google Scholar 

  • Sato, H., 1966. Induction of mutations in rice by some chemicals. Gamma Field Symp. 5: 71–81.

    Google Scholar 

  • Sato, H., 1966. A speaking in the panel discussion in the 5th Gamma Field Symposia held at Ohmiyamachi, lbaraki-ken, Japan.

  • Sato, J., 1966. Fundamentals of evolution theory. p. 331. Shokabo, Tokyo.

    Google Scholar 

  • Satpathy, D. & T. J. Arnason, 1969. Post-treatment temperature effects on mutation frequency in EMS-treated barley. Can. J. Genet. Cytol. 11: 522–530.

    Google Scholar 

  • Scholz, F., 1970. Utilization of induced mutations in barley. In: Barley genetics II. pp. 94–105. Washington State University Press, Washington.

    Google Scholar 

  • Siddiq, E. A. & M. S. Swaminathan, 1968. Induced mutations in relation to the breeding and phylogenetic differentiation of Oryza sativa. In: Rice breeding with induced mutations. pp. 25–51. IAEA, Vienna.

    Google Scholar 

  • Sigurbjornsson, B. & A. Mick, 1969. Progress in mutation breeding. In: Induced mutations in plants. pp. 673–698. IAEA. Vienna.

    Google Scholar 

  • Swaminathan, M. S. & N. P. Sarma, 1968. Alteration of the mutation spectrum in barley through treatments at different periods in the S phase of DNA synthesis. Current Sci. 37: 685–686.

    Google Scholar 

  • Swamudo, K., M. Tominaga, T. Kawase & K. Hayashi, 1952. Studies on the quantitative inheritance (8) A. Rice (Oryza Sativa L.) (a) Inheritance of the length of panicles and the quantitative function of the casual genes in their length determination. (4) On the quantitative function of the two multiple genes and the dwarfisch gene d1. Japan. J. Breed. 1: 254–260.

    Google Scholar 

  • Yamagata, H. & K. Syakudo, 1960. Studies on the utility of artificial mutations in plant breeding. I. γ-ray sensitivity of rice and induced aberrant plants in the X2 generation. Japan. J. Breed. 10: 153–162.

    Google Scholar 

  • Yamagata, H., 1964. Studies on induction and utilization of artificial mutations in rice. p. 239. Ph. D. Thesis submitted to Kyoto University, Kyoto.

  • Yamagata, H., K. Syakudo & T. Furukawa, 1965. Studies on the utility of artificial mutations in plant breeding. V. Mutagenic effects of several chemicals on rice. Japan. J. Breed. 15: 263–270.

    Google Scholar 

  • Yamaguchi, H., 1956. The biological action of X-ray on the rice plant of the different seed-grown origins. Japan. J. Breed. 6: 91–95.

    Google Scholar 

  • Yamaguchi, H., 1957. Comparison of the effects of mustards and X-rays on the mutation of rice. Japan. J. Breed. 7: 179–185.

    Google Scholar 

  • Yamaguchi, H., 1961. The procedure for estimating the frequency mutations. Japan. J. Breed. 11: 53–58.

    Google Scholar 

  • Yamaguchi, H., 1962. The effects of post-treatments with cysteine and sodium hydrosulfite on radiation-induced injury and mutation in rice. Japan. J. Breed. 12: 8–12.

    Google Scholar 

  • Yamaguchi, H., 1963. The methods for determining the mutation frequency after seed irradiation in rice. Journal of Radiation Research 4: 97–104.

    Google Scholar 

  • Yamaguchi, H., 1966. A review on mutation breeding researchs in Japan. Radioisotopes 15: 240–253.

    Google Scholar 

  • Yonezawa, K., Y. Yasumuro & K. Syakudo, 1969. The efficiency of mutation method in breeding of self-pollinated crops. Japan. J. Breed. 19: 159–170.

    Google Scholar 

  • Yonezawa, K., M. Ichii & Yamagata, 1971. Theoretical basis for determining the number of X2-plants required for improvement of quantitative characters of self-pollinating crops. II. Complement to the theory and numerical computations. Japan. J. Breed. 21: 160–168.

    Google Scholar 

  • Yonezawa, K., H. Yamagata & T. Tanisaka, 1973. Practical merit of delayed selections after single and recurrent mutagenic treatments. I. General considerations based on changes in mutant frequency and heritability. Radiation Botany 13: 1–12.

    Google Scholar 

  • Yonezawa, K. & H. Yamagata, 1975. Comparison of the scoring methods for mutation frequency in self-pollinating disomic plants. Radiation Botany 15: 241–256.

    Google Scholar 

  • Yonezawa, K., 1975. Method and efficiency of mutation breeding for quantitative characters. Gamma Field Symp. 14 (in press).

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Yonezawa, K., Yamagata, H. On the optimum mutation rate and optimum dose for practical mutation breeding. Euphytica 26, 413–426 (1977). https://doi.org/10.1007/BF00027003

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