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In Vivo Whole-Cell Recordings

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Advanced Patch-Clamp Analysis for Neuroscientists

Part of the book series: Neuromethods ((NM,volume 113))

Abstract

The introduction of whole-cell, patch clamp recordings in vivo has allowed measuring the synaptic (excitatory and inhibitory) inputs and the spike output from molecularly or anatomically identified neurons. Combining this technique with two-photon microscopy also allows to optically target such recordings to the different subtypes of inhibitory cells (e.g., soma- and dendrite targeting), as well as to measure dendritic integration of synaptic inputs in vivo. Here we summarize the potentialities and describe the critical steps to successfully apply such an informative technique to the study of the physiology and plasticity of brain microcircuits in the living, intact brain.

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Author information

Authors and Affiliations

  1. Molecular Biology Department, Umeå University, Umeå, 90187, Sweden

    Bojana Kokinovic, Stylianos Papaioannou & Paolo Medini

  2. Integrative Medical Biology (IMB) Department, Umeå University, Umeå, 90187, Sweden

    Bojana Kokinovic, Stylianos Papaioannou & Paolo Medini

  3. Italian Institute of Technology, Neuroscience and Brain Technologies Department, via Morego, 30, Genova, 16163, Italy

    Bojana Kokinovic

Authors
  1. Bojana Kokinovic
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  2. Stylianos Papaioannou
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  3. Paolo Medini
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Corresponding author

Correspondence to Paolo Medini .

Editor information

Editors and Affiliations

  1. The Leslie and Susan Gonda Multidisciplinary Brain Research Center, The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel

    Alon Korngreen

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Kokinovic, B., Papaioannou, S., Medini, P. (2016). In Vivo Whole-Cell Recordings. In: Korngreen, A. (eds) Advanced Patch-Clamp Analysis for Neuroscientists. Neuromethods, vol 113. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3411-9_1

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  • DOI: https://doi.org/10.1007/978-1-4939-3411-9_1

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3409-6

  • Online ISBN: 978-1-4939-3411-9

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