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Assessment of Functional Norovirus Antibody Responses by Blocking Assay in Mice

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Vaccine Design

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1403))

Abstract

Norovirus (NoV)-specific serum antibodies bind to NoV-derived virus-like particles (VLPs) and block the binding of VLPs to the host cell attachment factors/receptors, histo-blood group antigens (HBGAs). Blocking antibodies in human sera have been associated with a protection from NoV infection and disease. Studies of experimental NoV VLP-based vaccines measure blocking antibodies in animal sera instead of a traditional virus neutralization assay. This chapter describes the methodology for analyzing blocking antibodies from NoV GII.4 VLP-immunized mouse sera. Protocol for obtaining mouse NoV GII.4-specific immune sera is described, followed by the detailed protocol for blocking assay using synthetic HBGAs.

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Acknowledgements

We thank all personnel of Vaccine Research Center, University of Tampere, for their support in this work, especially Ms. Leena Huhti for the VLP production and Ms. Marianne Karlsberg for excellent technical help.

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Authors and Affiliations

  1. Vaccine Research Center, University of Tampere Medical School, Biokatu 10, Tampere, 33520, Finland

    Maria Malm, Kirsi Tamminen & Vesna Blazevic

Authors
  1. Maria Malm
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  2. Kirsi Tamminen
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  3. Vesna Blazevic
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Corresponding author

Correspondence to Vesna Blazevic .

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Editors and Affiliations

  1. Lankenau Institute for Medical Research, Wynnewood, Pennsylvania, USA

    Sunil Thomas

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Malm, M., Tamminen, K., Blazevic, V. (2016). Assessment of Functional Norovirus Antibody Responses by Blocking Assay in Mice. In: Thomas, S. (eds) Vaccine Design. Methods in Molecular Biology, vol 1403. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3387-7_13

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  • DOI: https://doi.org/10.1007/978-1-4939-3387-7_13

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3385-3

  • Online ISBN: 978-1-4939-3387-7

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