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The Assembly Line Enzymology of Polyketide Biosynthesis

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Nonribosomal Peptide and Polyketide Biosynthesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1401))

Abstract

Polyketides are a structurally and functionally diverse family of bioactive natural products that have found widespread application as pharmaceuticals, agrochemicals, and veterinary medicines. In bacteria complex polyketides are biosynthesized by giant multifunctional megaenzymes, termed modular polyketide synthases (PKSs), which construct their products in a highly coordinated assembly line-like fashion from a pool of simple precursor substrates. Not only is the multifaceted enzymology of PKSs a fascinating target for study, but it also presents considerable opportunities for the reengineering of these systems affording access to functionally optimized unnatural natural products. Here we provide an introductory primer to modular polyketide synthase structure and function, and highlight recent advances in the characterization and exploitation of these systems.

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Authors and Affiliations

  1. School of Biochemistry, University of Bristol, Medical Sciences Building, University Walk, Bristol, BS8 1TD, UK

    Marisa Till & Paul R. Race

  2. BrisSynBio Synthetic Biology Research Centre, University of Bristol, Life Sciences Building, Tyndall Avenue, Bristol, BS8 1TQ, UK

    Marisa Till & Paul R. Race

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  1. Marisa Till
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  1. Donald Danforth Plant Science Center, Saint Louis, Missouri, USA

    Bradley S. Evans

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Till, M., Race, P.R. (2016). The Assembly Line Enzymology of Polyketide Biosynthesis. In: Evans, B. (eds) Nonribosomal Peptide and Polyketide Biosynthesis. Methods in Molecular Biology, vol 1401. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3375-4_2

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  • DOI: https://doi.org/10.1007/978-1-4939-3375-4_2

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