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Real-Time PCR-Based Quantitation Method for the Genetically Modified Soybean Line GTS 40-3-2

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Recombinant Proteins from Plants

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1385))

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Abstract

This chapter describes a real-time PCR-based method for quantitation of the relative amount of genetically modified (GM) soybean line GTS 40-3-2 [Roundup Ready® soybean (RRS)] contained in a batch. The method targets a taxon-specific soybean gene (lectin gene, Le1) and the specific DNA construct junction region between the Petunia hybrida chloroplast transit peptide sequence and the Agrobacterium 5-enolpyruvylshikimate-3-phosphate synthase gene (epsps) sequence present in GTS 40-3-2. The method employs plasmid pMulSL2 as a reference material in order to quantify the relative amount of GTS 40-3-2 in soybean samples using a conversion factor (Cf) equal to the ratio of the RRS-specific DNA to the taxon-specific DNA in representative genuine GTS 40-3-2 seeds.

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References

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Author information

Authors and Affiliations

  1. National Food Research Institute, National Agriculture and Food Research Organization, 2-1-12 Kannon-dai, Tsukuba, 305-8642, Japan

    Kazumi Kitta, Reona Takabatake & Junichi Mano

Authors
  1. Kazumi Kitta
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  2. Reona Takabatake
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  3. Junichi Mano
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Corresponding author

Correspondence to Kazumi Kitta .

Editor information

Editors and Affiliations

  1. Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada, London, Ontario, Canada

    Jacqueline MacDonald

  2. Department of Biology, Western University, London, Ontario, Canada

    Igor Kolotilin

  3. London, Ontario, Canada

    Rima Menassa

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Kitta, K., Takabatake, R., Mano, J. (2016). Real-Time PCR-Based Quantitation Method for the Genetically Modified Soybean Line GTS 40-3-2. In: MacDonald, J., Kolotilin, I., Menassa, R. (eds) Recombinant Proteins from Plants. Methods in Molecular Biology, vol 1385. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3289-4_17

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  • DOI: https://doi.org/10.1007/978-1-4939-3289-4_17

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3288-7

  • Online ISBN: 978-1-4939-3289-4

  • eBook Packages: Springer Protocols

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