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Non-Covalently Functionalized of Single-Walled Carbon Nanotubes by DSPE-PEG-PEI for SiRNA Delivery

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SiRNA Delivery Methods

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1364))

Abstract

The expression of a gene can be specifically downregulated by small interfering RNA (SiRNA). Modified carbon nanotubes (CNT) can be used to protect SiRNA and facilitate its entry into cells. Regardless of that, simple and efficient functionalization of CNT is lacking. Effective SiRNA delivery can be carried out using non-covalently functionalized CNT, where non-covalent (versus covalent) functionalization is simpler and more expeditious. Non-covalently functionalized single walled carbon nanotubes (SWCNT) that include a lipopolymer are described here. Polyethylenimine (PEI) conjugated to 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000] (DSPE-PEG) was generated and the products used to disperse CNT to form DSPE-PEG-PEI/CNT (DGI/C), an agent capable of facilitating SiRNA delivery to cells in vitro and organs and cells in vivo.

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Authors and Affiliations

  1. Department of Pathology, Surgery, and Oncology, University of Western Ontario, Windermere Road, University Hospital C8-111, London, ON, N6A 5A5, Canada

    King Sun Siu, Xiufen Zheng, James Koropatnick & Wei-Ping Min

  2. London Regional Cancer Program, Lawson Health Research Institute, London, ON, Canada

    King Sun Siu, Xiufen Zheng, James Koropatnick & Wei-Ping Min

  3. Institute of Immunotherapy, Nanchang University and Jiangxi Academy of Medical Sciences, Nanchang, China

    Yujuan Zhang & Wei-Ping Min

Authors
  1. King Sun Siu
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  2. Yujuan Zhang
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  3. Xiufen Zheng
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  4. James Koropatnick
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  5. Wei-Ping Min
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Corresponding author

Correspondence to Wei-Ping Min .

Editor information

Editors and Affiliations

  1. Beckman Research Institute of City of Hope, Duarte, California, USA

    Kato Shum

  2. Beckman Research Institute of City of Hope, Duarte, California, USA

    John Rossi

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Siu, K.S., Zhang, Y., Zheng, X., Koropatnick, J., Min, WP. (2016). Non-Covalently Functionalized of Single-Walled Carbon Nanotubes by DSPE-PEG-PEI for SiRNA Delivery. In: Shum, K., Rossi, J. (eds) SiRNA Delivery Methods. Methods in Molecular Biology, vol 1364. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3112-5_13

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  • DOI: https://doi.org/10.1007/978-1-4939-3112-5_13

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3111-8

  • Online ISBN: 978-1-4939-3112-5

  • eBook Packages: Springer Protocols

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