Abstract
A considerable amount of work has been undertaken to genetically enhance the efficacy of baculovirus insecticides. Following construction of a genetically altered baculovirus, laboratory bioassays are used to quantify various parameters of insecticidal activity such as the median lethal concentration (or dose) required to kill 50 % of infected larvae (LC50 or LD50), median survival of larvae infected (ST50), and feeding damage incurred by infected larvae. In this chapter, protocols are described for a variety of bioassays and the corresponding data analyses for assessment of the insecticidal activity of baculovirus insecticides.
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Acknowledgements
The authors would like to thank Drs. Loy Volkman and Jan Washburn for training HL in accurate staging of larvae and use of the microapplicator and Steve Cooper for his assistance with photography. This material is based upon work supported by the Cooperative State Research, Education, and Extension Service, US Department of Agriculture, under Agreement No. 2003-35302-13558 as well as Hatch Act and State of Iowa funds. The US Department of Agriculture (USDA) prohibits discrimination in all its programs and activities on the basis of race, color, national origin, age, disability, and where applicable, sex, marital status, familial status, parental status, religion, sexual orientation, genetic information, political beliefs, reprisal, or because all or part of an individual’s income is derived from any public assistance program. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture.
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Popham, H.J.R., Ellersieck, M.R., Li, H., Bonning, B.C. (2016). Evaluation of the Insecticidal Efficacy of Wild Type and Recombinant Baculoviruses. In: Murhammer, D. (eds) Baculovirus and Insect Cell Expression Protocols. Methods in Molecular Biology, vol 1350. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3043-2_21
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DOI: https://doi.org/10.1007/978-1-4939-3043-2_21
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3042-5
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