Abstract
Somatic cell nuclear transfer (SCNT) is an exceptional experimental biology technique with an arguably great contribution to our current understanding of developmental plasticity. Many students and young researchers are interested in taking advantage of SCNT virtues in their experiments but the cost of micromanipulation microscopes, intensive training programs, and also the sophisticated process of SCNT may dissuade them from entering this amazing field of science. Here, we describe the details of a streamlined manual method of SCNT that can be performed using very basic equipment found in every embryology laboratory: the Pasteur pipette and stereomicroscope. The overall method introduced is very simple and a person with no previous experience in cloning can learn and adopt the basic routines of this technique independently.
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References
Spemann H (1938) Embryonic development and induction. Hafner Publishing Co, New York
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Acknowledgment
This study was supported by a Royan Institute grant for the Advancement of Reproductive Biotechnology. The Authors would like to thank the staff of the Embryology Department of Royan Institute for kind help in this project.
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1 Electronic Supplementary Material
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Blind manual oocyte enucleation (BME) (MP4 2167 kb)
Oriented manual oocyte enucleation (OME) (MP4 3396 kb)
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Hosseini, S.M., Moulavi, F., Nasr-Esfahani, M.H. (2015). A Novel Method of Somatic Cell Nuclear Transfer with Minimum Equipment. In: Verma, P., Sumer, H. (eds) Cell Reprogramming. Methods in Molecular Biology, vol 1330. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2848-4_15
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DOI: https://doi.org/10.1007/978-1-4939-2848-4_15
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2847-7
Online ISBN: 978-1-4939-2848-4
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