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Label-Free Cell Phenotypic Identification of Active Compounds in Traditional Chinese Medicines

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Label-Free Biosensor Methods in Drug Discovery

Part of the book series: Methods in Pharmacology and Toxicology ((MIPT))

Abstract

Traditional Chinese medicines (TCMs) have been used in clinic for thousands of years. These TCMs display reliable therapeutic efficacy and are important resources for drug discovery. Elucidating mechanisms of action (MOAs) of active compounds is essential to the development and clarification of TCMs. As one of new generation pharmacological assays, label-free cell phenotypic assays can provide a holistic view of ligand–receptor interactions in living cells with wide pathway coverage, high throughput, and high temporal resolution, thus enabling effectively elucidating the MOAs of TCMs. For identifying active compounds from TCMs, effective separation and purification methods are indispensable since TCMs usually contain hundreds or even thousands of compounds. This chapter provides a general protocol of preparative techniques and label-free cell phenotypic assays to determine the target engagement of active TCM fractions and compounds.

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Authors and Affiliations

  1. Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, Liaoning, 116023, China

    Xinmiao Liang, Jixia Wang & Xiuli Zhang

  2. Biochemical Technologies, Science and Technology Division, Corning Incorporated, Corning, NY, 14831, USA

    Ye Fang

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  1. Xinmiao Liang
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  2. Jixia Wang
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  3. Xiuli Zhang
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Correspondence to Xinmiao Liang .

Editor information

Editors and Affiliations

  1. Biochemical Technologies, Science and Technology Division, Corning Incorporated, Corning, New York, USA

    Ye Fang

6 Glossary

TCM

Traditional Chinese medicine

MOA

Mechanism of action

FDA

Food and Drug Administration

HPLC

High-performance liquid chromatography

2D-LC

Two-dimensional liquid chromatography

RPLC

Reversed-phase liquid chromatography

HILIC

Hydrophilic interaction liquid chromatography

IEX

Ion-exchange chromatography

FMCs

Fine multicomponents

ADME/Tox

    Absorption, distribution, metabolism, excretion and toxicity

RWG

Resonant waveguide grating

DMR

Dynamic mass redistribution

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Liang, X., Wang, J., Zhang, X., Fang, Y. (2015). Label-Free Cell Phenotypic Identification of Active Compounds in Traditional Chinese Medicines. In: Fang, Y. (eds) Label-Free Biosensor Methods in Drug Discovery. Methods in Pharmacology and Toxicology. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2617-6_13

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  • DOI: https://doi.org/10.1007/978-1-4939-2617-6_13

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2616-9

  • Online ISBN: 978-1-4939-2617-6

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