Abstract
The mitochondrial membrane potential is a critical parameter for understanding mitochondrial function, but it is challenging to quantitate with current methodologies which are based on the accumulation of cation indicators. Recently we have introduced a new methodology based on the redox poise of the b-hemes of the bc 1 complex. Here we describe the thermodynamic framework and algorithms necessary to calculate the membrane potential from the measured oxidation states of the b-hemes.
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Springett, R. (2015). Novel Methods for Measuring the Mitochondrial Membrane Potential. In: Weissig, V., Edeas, M. (eds) Mitochondrial Medicine. Methods in Molecular Biology, vol 1264. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2257-4_18
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DOI: https://doi.org/10.1007/978-1-4939-2257-4_18
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-2257-4
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