Abstract
Mammalian protein expression systems are becoming increasingly popular for the production of eukaryotic secreted and cell surface proteins. Here we describe methods to produce recombinant proteins in adherent or suspension human embryonic kidney cell cultures, using transient transfection or stable cell lines. The protocols are easy to scale up and cost-efficient, making them suitable for protein crystallization projects and other applications that require high protein yields.
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Seiradake, E., Zhao, Y., Lu, W., Aricescu, A.R., Jones, E.Y. (2015). Production of Cell Surface and Secreted Glycoproteins in Mammalian Cells. In: Owens, R. (eds) Structural Proteomics. Methods in Molecular Biology, vol 1261. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2230-7_6
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DOI: https://doi.org/10.1007/978-1-4939-2230-7_6
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