Structural Proteomics

High-Throughput Methods

  • Raymond J. Owens

Part of the Methods in Molecular Biology book series (MIMB, volume 1261)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Bioinformatics and Data Management

    1. Front Matter
      Pages 1-1
    2. Margaret J. Gabanyi, Helen M. Berman
      Pages 3-20
    3. Marco Punta, István Simon, Zsuzsanna Dosztányi
      Pages 35-59
  3. Soluble Proteins

    1. Front Matter
      Pages 61-61
    2. Matthias Haffke, Martin Marek, Martin Pelosse, Marie-Laure Diebold, Uwe Schlattner, Imre Berger et al.
      Pages 63-89
    3. Wassim Abdulrahman, Laura Radu, Frederic Garzoni, Olga Kolesnikova, Kapil Gupta, Judit Osz-Papai et al.
      Pages 91-114
    4. Elena Seiradake, Yuguang Zhao, Weixian Lu, A. Radu Aricescu, E. Yvonne Jones
      Pages 115-127
    5. Andreas K. Brödel, Doreen A. Wüstenhagen, Stefan Kubick
      Pages 129-140
    6. Vincent J. Fazio, Thomas S. Peat, Janet Newman
      Pages 141-156
  4. Membrane Proteins

    1. Front Matter
      Pages 157-157
    2. Erika Orbán, Davide Proverbio, Stefan Haberstock, Volker Dötsch, Frank Bernhard
      Pages 171-195
    3. Nien-Jen Hu, Heather Rada, Nahid Rahman, Joanne E. Nettleship, Louise Bird, So Iwata et al.
      Pages 197-209
    4. Isabel Moraes, Margarida Archer
      Pages 211-230
  5. Structural Characterization of Proteins

    1. Front Matter
      Pages 231-231
    2. Pierre Aller, Juan Sanchez-Weatherby, James Foadi, Graeme Winter, Carina M. C. Lobley, Danny Axford et al.
      Pages 233-253
    3. Giuliano Siligardi, Rohanah Hussain
      Pages 255-276

About this book

Introduction

This updated and expanded volume reflects the current state of the structural protein field with improved and refined protocols that have been applied to particularly challenging proteins, notably integral membrane proteins and multi-protein complexes. Structural Proteomics: High-Throughput Methods, Second Edition begins by exploring the resources available for curation, annotation, and structure prediction in silico, and continues with methods for sample preparation of both proteins and crystals, as well as structural characterization techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and up-to-date, Structural Proteomics: High-Throughput Methods, Second Edition will aid researchers in expanding our knowledge of this vital and expansive area of protein science.

Keywords

Bioinformatics Membrane proteins Multi-protein complexes Protein crystallization Protein structure analysis Soluble proteins Structural biology

Editors and affiliations

  • Raymond J. Owens
    • 1
  1. 1.Oxford Protein Production Facility UKDidcotUnited Kingdom

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-2230-7
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2229-1
  • Online ISBN 978-1-4939-2230-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book