Abstract
Neuronal apoptosis represents an intrinsic «suicide» program, by which a neuron orchestrates its own destruction. Although engagement of apoptosis requires transcription and protein synthesis , the complete spectrum of genes involved in distinct temporal domains remained unknown until the advent of genomics . In the last 10 years, the genome sequences and the development of high-throughput genomic technologies , such as DNA microarrays , have offered the unprecedented experimental opportunities to explore the transcriptional mechanisms underlying apoptosis from a new systems-level perspective. This review goes over this genomic approach and illustrates the use of microarray methodology to dissecting the multigenic program underlying neuronal apoptosis.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Arends MJ, Wyllie AH (1991) Apoptosis: mechanisms and roles in pathology. Int Rev Exp Pathol 32:223–254
Jellinger KA (2006) Challenges in neuronal apoptosis. Curr Alzheimer Res 3:377–391
Oppenheim RW (1991) Cell death during development of the nervous system. Annu Rev Neurosci 14:453–501
Pettmann B, Henderson CE (1998) Neuronal cell death. Neuron 20:633–647
Mattson MP (2006) Neuronal life-and-death signaling, apoptosis, and neurodegenerative disorders. Antioxid Redox Signal 8:1997–2006
Cavallaro S, Calissano P (2006) A genomic approach to investigate neuronal apoptosis. Curr Alzheimer Res 3:285–296
Cavallaro S, D’Agata V, Alessi E et al (2004) Gene expression profiles of apoptotic neurons. Genomics 84:485–496
Cavallaro S (2007) Neuronal apoptosis revealed by genomic analysis: integrating gene expression profiles with functional information. Neuroinformatics 5:115–126
Paratore S, Parenti R, Torrisi A et al (2006) Genomic profiling of cortical neurons following exposure to beta-amyloid. Genomics 88:468–479
Kristiansen M, Menghi F, Hughes R et al (2011) Global analysis of gene expression in NGF-deprived sympathetic neurons identifies molecular pathways associated with cell death. BMC Genomics 12:551
Ohkawara T, Nagase H, Koh CS et al (2011) The amyloid precursor protein intracellular domain alters gene expression and induces neuron-specific apoptosis. Gene 475:1–9
Desagher S, Severac D, Lipkin A et al (2005) Genes regulated in neurons undergoing transcription-dependent apoptosis belong to signaling pathways rather than the apoptotic machinery. J Biol Chem 280:5693–5702
Koh CH, Peng ZF, Ou K et al (2007) Neuronal apoptosis mediated by inhibition of intracellular cholesterol transport: microarray and proteomics analyses in cultured murine cortical neurons. J Cell Physiol 211:63–87
Kondo M, Shibata T, Kumagai T et al (2002) 15-Deoxy-delta(12,14)-prostaglandin J(2): the endogenous electrophile that induces neuronal apoptosis. Proc Natl Acad Sci U S A 99:7367–7372
Ng JM, Chen MJ, Leung JY et al (2012) Transcriptional insights on the regenerative mechanics of axotomized neurons in vitro. J Cell Mol Med 16:789–811
Choy MS, Chen MJ, Manikandan J et al (2011) Up-regulation of endoplasmic reticulum stress-related genes during the early phase of treatment of cultured cortical neurons by the proteasomal inhibitor lactacystin. J Cell Physiol 226:494–510
D’Mello SR, Galli C, Ciotti T et al (1993) Induction of apoptosis in cerebellar granule neurons by low potassium: inhibition of death by insulin-like growth factor I and cAMP. Proc Natl Acad Sci U S A 90:10989–10993
Galli C, Meucci O, Scorziello A et al (1995) Apoptosis in cerebellar granule cells is blocked by high KCl, forskolin, and IGF-1 through distinct mechanisms of action: the involvement of intracellular calcium and RNA synthesis. J Neurosci 15:1172–1179
Cavallaro S, Copani A, D’Agata V et al (1996) Pituitary adenylate cyclase activating polypeptide prevents apoptosis in cultured cerebellar granule neurons. Mol Pharmacol 50:60–66
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2015 Springer Science+Business Media New York
About this protocol
Cite this protocol
Cavallaro, S. (2015). Genomic Analysis of Transcriptional Changes Underlying Neuronal Apoptosis . In: Lossi, L., Merighi, A. (eds) Neuronal Cell Death. Methods in Molecular Biology, vol 1254. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2152-2_11
Download citation
DOI: https://doi.org/10.1007/978-1-4939-2152-2_11
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2151-5
Online ISBN: 978-1-4939-2152-2
eBook Packages: Springer Protocols