Abstract
This study was performed to identify pathological fungi of eight species [Aspergillus fumigatus, Candida albicans, Torulopsis (Candida) glabrata, Cryptococcus neoformans, Fusarium anthophilum, Rhizopus oryzae, Sporothrix schenckii and Trichosporon beigelii] in formalin-fixed, paraffin-embedded tissue sections by indirect immunoperoxidase staining. Mature albino rabbits were immunized with formalin-killed organisms. Antibodies were prepared by precipitation. Immunoperoxidase staining was applied to the paraffin-embedded tissue sections of experimentally infected mice and human autopsy and surgical specimens. Although the cell walls of each fungus stained clearly, many cross-reactivities appeared. However, it was possible to obtain specificity for the eight species by absorption and dilution of the antisera.
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References
Chmel H (1993) Fungal infections in the immunocompromised host. Clinical syndromes and diagnosis. In: Murphy JW, Friedman H, Bendinelli M (eds) Fungal infections and immune responses. Plenum Press, New York, pp 405–424
DeGregorio MW, Lee WM, Linker CA, Jacobs RA, Ries CA (1982) Fungal infections in patients with acute leukemia. Am J Med 73:543–548
Fleet GH (1991) Cell walls. In: Rose AH, Harrison JS (eds) The yeast, 2nd edn, vol 4, Academic Press, London, pp 206–238
Ho YM, Ng MH, Huang CT (1979) Antibodies to germinating and yeast cells of Candida albicans in human and rabbit sera. J Clin Pathol 32:399–405
Hotchi M (1967) The application of fluorescent antibody techniques to the identification of pathogenic fungi in tissue specimens. Med J Shinshu Univ 12:123–139
Hotchi M (1991) Pathological diagnosis of mycosis (in Japanese) Pathol Clin Med 9:1139–1147
Hotchi M, Schwarz J, Kaplan W (1972) Limitations of fluorescent antibody staining of Histoplasma capsulatum in tissue sections. Sabouraudia 10:157–163
Hotchi M, Okada M, Nasu T (1980) Present state of fungal infections in autopsy cases in Japan. Am J Clin Pathol 74:410–416
Kaneko T, Kato T, Cui J, Abe F, Hotchi M (1987) Deep-seated mycoses and tissue lesions in recent autopsy cases (in Japanese) Jpn J Med Mycol 28:241–249
Kaplan W, Kraft DE (1969) Demonstration of pathogenic fungi in formalin-fixed tissues by immunofluorescence. Am J Clin Pathol 52:420–432
Kauffman HF, Vries K de (1980) Antibodies against Aspergillus fumigatus. I. Standardization of the antigenic composition. Int Arch Allergy Appl Immunol 62:252–264
Kaufman L (1992) Immunohistologic diagnosis of systemic mycoses: an update. Eur J Epidemiol 8:377–382
Kimura M, Takahashi H, Satou T, Hashimoto S (1989) An autopsy case of disseminated trichosporonosis with candidiasis of the urinary bladder. Virchows Arch [A] 416:159–162
Kobayashi K (1984) Histopathological studies on experimental pulmonary aspergillosis (in Japanese) Shinshu Med J 32:43–61
Kobayashi K, Hayama M, Hotchi M (1988) The application of immunoperoxidase staining for the detection of causative fungi in tissue specimens of mycosis. I. Mycopathologia 102:107–113
Melcher GP, Rinaldi MG, Frey CL, Drutz DJ (1988) Demonstration, by immunoelectron microscopy, of a cell wall antigen in Trichosporon beigelii that cross-reacts with Cryptococcus neoformans capsular polysaccharide. J Infect Dis 158:901–902
Moskowitz LB, Ganjei P, Ziegels-Weissman J, Cleary TJ, Penneys NS, Nadji M (1986) Immunohistologic identification of fungi in systemic and cutaneous mycoses. Arch Pathol Lab Med 110:433–436
Nakamura T (1984) Experimental pulmonary candidiasis in modified rabbits. Histopathological, ultrastructural and enzyme cytochemical studies of tissue reactions. Mycopathologia 85:129–144
Nakane PK (1975) Recent progress in the peroxidase-labeled antibody method. Ann NY Acad Sci 254:203–211
Nakane PK, Pierce GB Jr (1967) Enzyme-labeled antibodies for the light and electron microscopic localization of tissue antigens. J Cell Biol 33:307–318
Pierard GE, Estrada JA, Pierard-Franchimont C, Thiry A, Stynen D (1991) Immunohistochemical expression of galactomannan in the cytoplasm of phagocytic cells during invasive aspergillosis. Am J Clin Pathol 96:373–376
Reed JA, Hemann BA, Alexander JL, Brigati DJ (1993) Immunomycology: Rapid and specific immunocytochemical identification and fungi in formalin-fixed, paraffin-embedded material. J Histochem Cytochem 41:1217–1221
Saeed EN, Hay RJ (1981) Immunoperoxidase staining in the recognition of Aspergillus infections. Histopathology 5:437–444
Sundstrom PM, Kenny GE (1984) Characterization of antigens specific to the surface of germ tubes of Candida albicans by immunofluorescence. Infect Immun 43:850–855
Westhal OL, Luderitz O, Bister F (1952) Über die Extraktion von Bakterien mit Phenol/Wasser. Z Naturforsch 7:148–155
Yamaguchi H, Miyaji M, Nishimura K (1987) Biology of pathogenic fungi, (in Japanes). Nanzando, Tokyo, pp 81–88
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Fukuzawa, M., Inaba, H., Hayama, M. et al. Improved detection of medically important fungi by immunoperoxidase staining with polyclonal antibodies. Vichows Archiv A Pathol Anat 427, 407–414 (1995). https://doi.org/10.1007/BF00199390
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DOI: https://doi.org/10.1007/BF00199390