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Cofactor Profiling of the Glucocorticoid Receptor from a Cellular Environment

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Steroid Receptors

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1204))

Abstract

The Microarray Assay for Realtime Coregulator-Nuclear receptor Interaction (MARCoNI) technology allows the identification of nuclear receptor–coregulator interactions via flow-through microarrays. As such, differences in the coregulator profile between distinct nuclear receptors or of a single receptor in agonist or antagonist mode can be investigated, even in a single run. In this chapter, the method how to perform these peptide microarrays with cell lysates containing the overexpressed glucocorticoid receptor is described, as well as the influence of assay parameters, variations to the protocol, and data analysis.

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References

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Author information

Authors and Affiliations

  1. Cytokine Receptor Lab, VIB Department of Medical Protein Research, Department of Biochemistry, University of Ghent, Albert Baertsoenkaai 3, 9000, Ghent, Belgium

    Sofie J. Desmet, Jonathan Thommis & Karolien De Bosscher

  2. Mouse Genetics in Inflammation, VIB Inflammation Research Center, Ghent University, Ghent, Belgium

    Lien Dejager & Claude Libert

  3. Department Medicine & Health Sciences - Laboratory of Experimental Cancer Research, Ghent University, Ghent, Belgium

    Dorien Clarisse & Ilse M. Beck

  4. PamGene International B.V., ‘s Hertogenbosch, Den Bosch, The Netherlands

    Diana Melchers, Niek Bastiaensen, Rob Ruijtenbeek & René Houtman

  5. Department of Endocrinology, Leiden University Medical Centre, ZA Leiden, The Netherlands

    Onno C. Meijer

Authors
  1. Sofie J. Desmet
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  2. Lien Dejager
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  3. Dorien Clarisse
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  4. Jonathan Thommis
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  5. Diana Melchers
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  6. Niek Bastiaensen
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  7. Rob Ruijtenbeek
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  8. Ilse M. Beck
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  9. Claude Libert
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  10. René Houtman
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  11. Onno C. Meijer
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  12. Karolien De Bosscher
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Corresponding author

Correspondence to Karolien De Bosscher .

Editor information

Editors and Affiliations

  1. Second University of Naples, Naples, Italy

    Gabriella Castoria  & Ferdinando Auricchio  & 

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© 2014 Springer Science+Business Media New York

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Cite this protocol

Desmet, S.J. et al. (2014). Cofactor Profiling of the Glucocorticoid Receptor from a Cellular Environment. In: Castoria, G., Auricchio, F. (eds) Steroid Receptors. Methods in Molecular Biology, vol 1204. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-1346-6_8

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  • DOI: https://doi.org/10.1007/978-1-4939-1346-6_8

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-1345-9

  • Online ISBN: 978-1-4939-1346-6

  • eBook Packages: Springer Protocols

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