Steroid Receptors

Methods and Protocols

  • Gabriella Castoria
  • Ferdinando Auricchio

Part of the Methods in Molecular Biology book series (MIMB, volume 1204)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Guillermo P. Vicent, A. Silvina Nacht, Cecilia Ballaré, Roser Zaurin, Daniel Soronellas, Miguel Beato
    Pages 1-14
  3. Rosaria Benedetti, Mariarosaria Conte, Vincenzo Carafa, Bartolomeo Della Ventura, Carlo Altucci, Raffaele Velotta et al.
    Pages 25-34
  4. Nicoletta Rizzi, Balaji Ramachandran, Cristina Vantaggiato, Paolo Ciana, Adriana Maggi
    Pages 45-58
  5. Sofie J. Desmet, Lien Dejager, Dorien Clarisse, Jonathan Thommis, Diana Melchers, Niek Bastiaensen et al.
    Pages 83-94
  6. Stephen R. Hammes, Susanne U. Miedlich, Aritro Sen
    Pages 95-108
  7. Pia Giovannelli, Marzia Di Donato, Ferdinando Auricchio, Gabriella Castoria
    Pages 109-121
  8. Cheryl S. Watson, Yow-Jiun Jeng, Nataliya N. Bulayeva, Celeste C. Finnerty, Luke Y. Koong, Dragoslaca Zivadinovic et al.
    Pages 123-133
  9. Antonio Bilancio, Antimo Migliaccio
    Pages 145-153
  10. Stefania Catalano, Ines Barone, Sebastiano Andò
    Pages 155-163
  11. Caterina De Rosa, Valentina Rossi, Ciro Abbondanza
    Pages 165-185
  12. Yamil R. Yusef, Warren Thomas, Brian J. Harvey
    Pages 219-231

About this book

Introduction

Steroid Receptors: Methods and Protocols presents a selection of techniques that have been recently applied to the analysis of steroid receptors, powerful tools for the advancement of our understanding of both the mechanisms regulating gene transcription and the rapid signaling responses of tissues to signals. Research in this area has generated a wealth of data allowing the elucidation of steroid receptor mechanisms and improving the treatment of many endocrine disorders, above all cancers. Chapters cover methods to analyze gene transcription, chromatin and proteomic modifications, extra-nuclear signaling regulation, development of cell and animal models, and preparation of new antibodies.  Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Steroid Receptors: Methods and Protocols offers an updated view on a variety of modern methods that will hopefully contribute to improving our knowledge on the integration of steroid receptors in single or different functionally connected cellular contexts under a variety of physiological and pathological conditions, above all tumors.

Keywords

chromatin gene transcription proteomic modifications steroid receptors steroid signaling

Editors and affiliations

  • Gabriella Castoria
    • 1
  • Ferdinando Auricchio
    • 2
  1. 1.Second University of NaplesNaplesItaly
  2. 2.Second University of NaplesNaplesItaly

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-1346-6
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-1345-9
  • Online ISBN 978-1-4939-1346-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book