Abstract
Cell therapy techniques are a promising option for tissue regeneration; especially in cases such as heart failure where transplantation is limited by donor availability. Multiple cell types have been examined for myocardial regeneration, including mesenchymal stem cells (and other bone marrow-derived cells), induced pluripotent stem cells, embryonic stem cells, cardiosphere-derived cells, and cardiac progenitor cells (CPCs). CPCs are multipotent and clonogenic, can be harvested from mature tissue, and have the distinct advantages of autologous transplant and lack of tumor formation in a clinical setting. Here we focus on the isolation, expansion, and myocardial differentiation of rat CPCs. Brief adaptations of the protocol for isolation from mouse and human tissue are also provided.
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Acknowledgments
This material is based upon work supported by the National Science Foundation Graduate Research Fellowship under Grant No. DGE-1148903 to KMF as well as a grant from the National Institutes of Health HL094527 to MED.
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French, K.M., Davis, M.E. (2014). Isolation and Expansion of C-Kit-Positive Cardiac Progenitor Cells by Magnetic Cell Sorting. In: Radisic, M., Black III, L. (eds) Cardiac Tissue Engineering. Methods in Molecular Biology, vol 1181. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1047-2_4
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DOI: https://doi.org/10.1007/978-1-4939-1047-2_4
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-1046-5
Online ISBN: 978-1-4939-1047-2
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