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Scalable Cardiac Differentiation of Human Pluripotent Stem Cells as Microwell-Generated, Size Controlled Three-Dimensional Aggregates

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Cardiac Tissue Engineering

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1181))

Abstract

The formation of cells into more physiologically relevant three-dimensional multicellular aggregates is an important technique for the differentiation and manipulation of stem cells and their progeny. As industrial and clinical applications for these cells increase, it will be necessary to execute this procedure in a readily scalable format. We present here a method employing microwells to generate large numbers of human pluripotent stem cell aggregates and control their subsequent differentiation towards a cardiac fate.

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Acknowledgements

We thank Dr. Peter Zandstra, in whose laboratory this protocol was developed, and Drs. Mark Gagliardi and Gordon Keller who provided assistance in establishing the initial methods on which this process was based. Protocol development was supported by an Ontario Graduate Scholarship in Science and Technology to C.B. and a grant from the Heart and Stroke Foundation of Ontario to Peter Zandstra.

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Authors and Affiliations

  1. Centre for Commercialization of Regenerative Medicine, 100 College Street, Room 508, Toronto, ON, M5G 1L5, Canada

    Celine L. Bauwens

  2. Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine, University of Calgary, HMRB 320, 3330 Hospital Drive NW, Calgary, AB, T2N 4N1, Canada

    Mark D. Ungrin

Authors
  1. Celine L. Bauwens
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  2. Mark D. Ungrin
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Corresponding author

Correspondence to Mark D. Ungrin .

Editor information

Editors and Affiliations

  1. Department Chemical Engineering&Applied Chemistry, University of Toronto, Toronto, Ontario, Canada

    Milica Radisic

  2. Department of Biomedical Engineering, Tufts University, Medford, Massachusetts, USA

    Lauren D. Black III

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Bauwens, C.L., Ungrin, M.D. (2014). Scalable Cardiac Differentiation of Human Pluripotent Stem Cells as Microwell-Generated, Size Controlled Three-Dimensional Aggregates. In: Radisic, M., Black III, L. (eds) Cardiac Tissue Engineering. Methods in Molecular Biology, vol 1181. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1047-2_2

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  • DOI: https://doi.org/10.1007/978-1-4939-1047-2_2

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1046-5

  • Online ISBN: 978-1-4939-1047-2

  • eBook Packages: Springer Protocols

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