Abstract
Conventional methods for the detection of Listeria monocytogenes in foods and environmental samples relies on selective pre-enrichment, enrichment, and plating. This is followed by confirmation of suspected colonies by testing a limited number of biochemical markers.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Hitchins AD, Jinneman K (2011) Detection and enumeration of Listeria monocytogenes in foods. In: Bacteriological analytical manual, Chapter 10. U.S. Food and Drug Administration. http://www.fda.gov/food/scienceresearch/laboratorymethods/bacteriologicalanalytical.manualbam/ucm071400.htm.Accessed 17 Mar 2013
USDA (2012) Isolation and identification of Listeria monocytogenes from red meat, poultry and egg products, and environmental samples. In: Microbiology laboratory guidebook, Method Number 8.08. United States Department of Agriculture Food Safety and Inspection Service, Office of Public Health Science. http://www.fsis.usda.gov/PDF/MLG-8.pdf. Accessed 17 Mar 2013
ISO (2004) ISO 11290-1. Microbiology of food and animal feeding stuffs—horizontal method for the detection and enumeration of Listeria monocytogenes—part 1: detection method amendment 1: modification of the isolation media and the haemolysis test and inclusion of precision data. International Organization for Standardization, Geneva
ISO (2004) ISO 11290-2. Microbiology of food and animal feeding stuffs—horizontal method for the detection and enumeration of Listeria monocytogenes—part 2: enumeration method amendment 1: modification of the enumeration medium. International Organization for Standardization, Geneva
EC (2005) Commission Regulation Nº 2073/2005of 15 November 2005 on microbiological criteria for foodstuffs. Official Journal of the European Union L338:1–26. http://eurlex.europa.eu/LexUriServ/LexUriServ.do?uri = OJ:L:2005:338:0001:0026:EN:PDF. Accessed 17 Mar 2013
Miller FA, Brandão TRS, Teixeira P et al (2006) Recovery of heat-injured Listeria innocua. Int J Food Microbiol 112:261–265
Montville TJ, Matthews KR (2008) Factors that influence microbes in foods. In: Montville TJ, Matthews KR (eds) Food microbiology: an introduction, 2nd edn. ASM Press, Washington, DC, pp 17–19
ISO (1996) ISO 11290-1. Microbiology of food and animal feeding stuffs—horizontal method for the detection and enumeration of Listeria monocytogenes—part 1: detection method. International Organization for Standardization, Geneva
ISO (1998) ISO 11290-2. Microbiology of food and animal feeding stuffs—horizontal method for the detection and enumeration of Listeria monocytogenes—part 2: enumeration method. International Organization for Standardization, Geneva
Johansson T (1998) Enhanced detection and enumeration of Listeria monocytogenes from foodstuffs and food-processing environments. Int J Food Microbiol 40:77–85
Reissbrodt R (2004) New chromogenic plating media for detection and enumeration of pathogenic Listeria spp.—an overview. Int J Food Microbiol 95:1–9
USDA (2008) Most probable number procedure and tables. In: Laboratory guidebook, Appendix 2.03. United States Department of Agriculture Food Safety and Inspection Service, Office of Public Health Science. http://www.fsis.usda.gov/PDF/MLG_Appendix_2_03.pdf. Accessed 17 Mar 2013
Lovette J, Frances DW, Hunt JM (1987) Listeria monocytogenes in raw milk: detection, incidence and pathogenicity. J Food Prot 50:188–192
Donnelly CW, Baigent GJ (1986) Method for flow cytometric detection of Listeria monocytogenes in milk. Appl Environ Microbiol 52:689–695
Fraser JA, Sperber WH (1988) Rapid detection of Listeria spp. in food and environmental samples by esculin hydrolysis. J Food Prot 51:762–765
van Netten P, Perales I, van de Moosalijk A, Curtis GDW, Mossel DAA (1989) Liquid and solid selective differential media for the detection and enumeration of L. monocytogenes and other Listeria spp. Int J Food Microbiol 8:299–317
Curtis GDW, Mitchell RG, King AF, Emma J (1989) A selective differential medium for the isolation of Listeria monocytogenes. Lett Appl Microbiol 8:95–98
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer Science+Business Media New York
About this protocol
Cite this protocol
Magalhães, R., Mena, C., Ferreira, V., Almeida, G., Silva, J., Teixeira, P. (2014). Traditional Methods for Isolation of Listeria monocytogenes . In: Jordan, K., Fox, E., Wagner, M. (eds) Listeria monocytogenes. Methods in Molecular Biology, vol 1157. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0703-8_2
Download citation
DOI: https://doi.org/10.1007/978-1-4939-0703-8_2
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-0702-1
Online ISBN: 978-1-4939-0703-8
eBook Packages: Springer Protocols