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Traditional Methods of Analysis for Listeria monocytogenes

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Listeria Monocytogenes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2220))

Abstract

Conventional methods for the detection of Listeria monocytogenes in foods and environmental samples rely on selective pre-enrichment, enrichment, and plating. This is followed by confirmation of suspected colonies by testing a limited number of biochemical markers.

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References

  1. Hitchins AD, Jinneman K, Chen Y (2017) BAM: Detection of Listeria monocytogenes in foods and environmental samples, and enumeration of Listeria monocytogenes in foods. In: Bacteriological Analytical Manual. https://www.fda.gov/food/laboratory-methods-food/bam-detection-and-enumeration-listeria-monocytogenes. Accessed 4 July 2019

  2. USDA (2019) Isolation and identification of Listeria monocytogenes from red meat, poultry and egg products, and environmental samples. In: Microbiology laboratory guidebook, Method Number 8.11. United States Department of Agriculture Food Safety And Inspection Service, Office of Public Health Science. https://www.fsis.usda.gov/wps/wcm/connect/1710bee8-76b9-4e6c-92fc-fdc290dbfa92/MLG-8.pdf?MOD=AJPERES. Accessed 4 July 2019

  3. ISO (2017a) ISO 11290-1. Microbiology of the food chain—horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp.—Part 1: Detection method. International Organization for Standardization, Geneva

    Google Scholar 

  4. ISO. (2017b). ISO 11290-2. Microbiology of the food chain. – horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. – Part 2: Enumeration method. International Organization for Standardization, Geneva

    Google Scholar 

  5. EC (2005) Commission regulation N° 2073/2005 of 15 November 2005 on microbiological criteria for foodstuffs. Off J Eur Union L338:1–26. https://eur-lex.europa.eu/legal-content/EN/TXT/?uri=OJ:L:2005:338:TOC. Accessed 4 July 2019

    Google Scholar 

  6. Miller FA, Brandão TRS, Teixeira P et al (2006) Recovery of heat-injured Listeria innocua. Int J Food Microbiol 112:261–265

    Article  CAS  Google Scholar 

  7. Montville TJ, Matthews KR (2008) Factors that influence microbes in foods. In: Food microbiology: an introduction, 2nd edn. ASM Press, Washington

    Google Scholar 

  8. ISO (1996) ISO 11290-1. Microbiology of food and animal feeding stuffs—horizontal method for the detection and enumeration of Listeria monocytogenes—part 1: detection method. International Organization for Standardization, Geneva

    Google Scholar 

  9. ISO (1998) ISO 11290-2. Microbiology of food and animal feeding stuffs—horizontal method for the detection and enumeration of Listeria monocytogenes—part 2: enumeration method. International Organization for Standardization, Geneva

    Google Scholar 

  10. Johansson T (1998) Enhanced detection and enumeration of Listeria monocytogenes from foodstuffs and food-processing environments. Int J Food Microbiol 40:77–85

    Article  CAS  Google Scholar 

  11. Reissbrodt R (2004) New chromogenic plating media for detection and enumeration of pathogenic Listeria spp.-an overview. Int J Food Microbiol 95:1–9

    Article  CAS  Google Scholar 

  12. ISO 7218/ (2007)/ Amd.1:2013. Microbiology of food and animal feeding stuffs—General requirements and guidance for microbiological examinations. International Organization for Standardization, Geneva. https://standards.iso.org/iso/7218/. Accessed 4 July 2019

  13. Lovette J, Frances DW, Hunt JM (1987) Listeria monocytogenes in raw milk: detection, incidence and pathogenicity. J Food Prot 50:188–192

    Article  Google Scholar 

  14. Donnelly CW, Baigent GJ (1986) Method for flow cytometric detection of Listeria monocytogenes in milk. Appl Environ Microbiol 52:689–695

    Article  CAS  Google Scholar 

  15. Fraser JA, Sperber WH (1988) Rapid detection of Listeria spp. in food and environmental samples by esculin hydrolysis. J Food Protect 51:762–765

    Article  Google Scholar 

  16. van Netten P, Perales I, van de Moosalijk A et al (1989) Liquid and solid selective differential media for the detection and enumeration of L. monocytogenes and other Listeria spp. Int J Food Microbiol 8:299–317

    Article  Google Scholar 

  17. Curtis GDW, Mitchell RG, King AF et al (1989) A selective differential medium for the isolation of Listeria monocytogenes. Lett Appl Microbiol 8:95–98

    Article  Google Scholar 

  18. Rodríguez-Lázaro D, Hernández M (2014) Confirmation of isolates of Listeria by conventional and real-time PCR. Methods Mol Biol 1157:31–38. https://doi.org/10.1007/978-1-4939-0703-8_3

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Correspondence to Paula Teixeira .

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Magalhães, R., Mena, C., Ferreira, V., Almeida, G., Silva, J., Teixeira, P. (2021). Traditional Methods of Analysis for Listeria monocytogenes. In: Fox, E.M., Bierne, H., Stessl, B. (eds) Listeria Monocytogenes. Methods in Molecular Biology, vol 2220. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0982-8_1

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  • DOI: https://doi.org/10.1007/978-1-0716-0982-8_1

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0981-1

  • Online ISBN: 978-1-0716-0982-8

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