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Therapeutic Microbes for Infectious Disease

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Engineering and Analyzing Multicellular Systems

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1151))

Abstract

The rapid emergence of multidrug-resistant pathogens has invoked concerns of our current limitations in controlling the spread of infectious disease. To resolve this, we have applied synthetic biology principles to engineer human commensal microbe that can specifically sense and kill an antibiotic-resistant strain of P. aeruginosa. In this chapter, we describe the methods used to assemble, characterize, and evaluate the effectiveness of our engineered microbe in multicellular systems.

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References

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Acknowledgements

This work was funded by the National Medical Research Council of Singapore (CBRG11nov109) and the National Research Foundation of Singapore (NRF-CRP5-2009-03). The first author wishes to dedicate this work to C.L.P., M.W.C., M.N., and S.T.

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Authors and Affiliations

  1. School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore, Singapore

    Choon Kit Wong, Mui Hua Tan, Bahareh Haji Rasouliha & Chueh Loo Poh

  2. Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore

    In Young Hwang, Hua Ling & Matthew Wook Chang

Authors
  1. Choon Kit Wong
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  2. Mui Hua Tan
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  3. Bahareh Haji Rasouliha
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  4. In Young Hwang
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  5. Hua Ling
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  6. Chueh Loo Poh
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  7. Matthew Wook Chang
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Corresponding author

Correspondence to Matthew Wook Chang .

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Editors and Affiliations

  1. School of Life Sciences, University of Science & Technology of China,, Hefei, Anhui, China

    Lianhong Sun

  2. Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA

    Wenying Shou

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© 2014 Springer Science+Business Media New York

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Wong, C.K. et al. (2014). Therapeutic Microbes for Infectious Disease. In: Sun, L., Shou, W. (eds) Engineering and Analyzing Multicellular Systems. Methods in Molecular Biology, vol 1151. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0554-6_9

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  • DOI: https://doi.org/10.1007/978-1-4939-0554-6_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0553-9

  • Online ISBN: 978-1-4939-0554-6

  • eBook Packages: Springer Protocols

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