Abstract
Transgenic mice are a beneficial tool that can allow researchers to investigate the roles of specific genes in physiology and disease. However, conventional transgenic mice have the limitation that constitutive expression of a transgene from the embryonic stage may affect the normal development of the mice or cause compensating effects. To overcome these disadvantages, tetracycline-controlled transgenic mice, which can express target gene products in a tissue-specific and time-dependent manner, have been developed. In this section, the principles of tetracycline-controlled systems are discussed first. In addition, useful information for generating transgenic mice using this system is introduced.
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Sakai, N. (2014). Principles for the Use of In Vivo Transgene Techniques: Overview and an Introductory Practical Guide for the Selection of Tetracycline-Controlled Transgenic Mice. In: Shiozawa, S. (eds) Arthritis Research. Methods in Molecular Biology, vol 1142. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0404-4_4
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DOI: https://doi.org/10.1007/978-1-4939-0404-4_4
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-0404-4
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