Abstract
Phage-assisted evolution has emerged as a powerful technique for improving a protein’s function by using mutagenesis and selective pressure. However, mutations typically occur throughout the host’s genome and are not limited to the gene-of-interest (GOI): these undesirable genomic mutations can yield host cells that circumvent the system’s selective pressure. Our system targets mutations specifically toward the GOI by combining T7 targeted mutagenesis and phage-assisted evolution. This system improves the structure and function of proteins by accumulating favorable mutations that can change its binding affinity, specificity, and activity.
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Acknowledgement
We are grateful for assistance from Afnan Khan and Montdher Hussain and for support from NSERC and the UTM Office of Research.
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Ali, M., Akel, R., Botero, M.J., Shin, J.A. (2024). Combining a Base Deaminase Mutator with Phage-Assisted Evolution. In: Peng, H., Liu, J., Chen, I.A. (eds) Phage Engineering and Analysis. Methods in Molecular Biology, vol 2793. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3798-2_4
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DOI: https://doi.org/10.1007/978-1-0716-3798-2_4
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