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Phage Engineering and Analysis

Methods and Protocols

  • Book
  • © 2024

Overview

Editors:
  1. Huan Peng

    1. Cellular Signaling Laboratory International Research, Center for Sensory Biology and Technology of MOST, Key Laboratory of Molecular Biophysics of MOE, College of Life Science and Technology, Huazhong University of Science and Technology (HUST), Wuhan, China

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  2. Jianfeng Liu

    1. College of Life Science and Technology, Huazhong University of Science and Technology (HUST), Wuhan, China

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  3. Irene A. Chen

    1. Department of Chemical and Biomolecular Engineering, Department of Chemistry and Biochemistry, University of California, Los Angeles, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2793)

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About this book

This volume explores the latest developments in the study and application of phage biology. The chapters in this book are divided into five parts and cover topics such as phage display, selection, and evolution; genetic and chemical modification of phages; analyzing structures by electron microscopy; characterizing phage transcripts and proteins; and the biology of whole phages. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Cutting-edge and comprehensive, Phage Engineering and Analysis: Methods and Protocols is a valuable resource that will help both expert and novice researchers - from backgrounds ranging from microbiologists to biophysicists to chemical engineers - further enhance their understandingof this important and evolving field.


Keywords

Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
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  2. Phage Display: Selection and Evolution

    1. Front Matter

      Pages 1-1
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    2. Protocols for Building and Producing High Diversity Peptide Phage Display Libraries

      • Ricardo Jose Giordano, Lilian Costa Alecrim
      Pages 3-19

    3. Generation of a Naïve Human scFv Phage Display Library and Panning Selection

      • Brenda Pei Chui Song, Jing Yi Lai, Theam Soon Lim
      Pages 21-40

    4. Structure-Guided and Phage-Assisted Evolution of Therapeutic Antibodies to Reverse On-Target Point Mutation-Mediated Resistance

      • Xinlei Zhuang, Shuqing Chen, Liqiang Pan
      Pages 41-54

    5. Combining a Base Deaminase Mutator with Phage-Assisted Evolution

      • Maryam Ali, Raneem Akel, Maria J. Botero, Jumi A. Shin
      Pages 55-64

    6. Understanding the Structural Requirements of Peptide–Protein Interaction and Applications for Peptidomimetic Development

      • Angy Liseth Davalos Macias, Lilian Costa Alecrim, Fabio C. L. Almeida, Ricardo Jose Giordano
      Pages 65-82

  3. Engineering Phages

    1. Front Matter

      Pages 83-83
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    2. Contracting the Host Range of Bacteriophage T7 Using a Continuous Evolution System

      • Tzvi Holtzman, Ram Nechooshtan, Ido Yosef, Udi Qimron
      Pages 85-100

    3. Single-Cell Proteomics by Barcoded Phage-Displayed Screening via an Integrated Microfluidic Chip

      • Yujiao Wang, Jing Zhao, Zhenwei Jiang, Yuan Ma, Rui Zhang
      Pages 101-112

    4. Targeted Genome Editing of Virulent Pseudomonas Phages Using CRISPR-Cas3

      • Kaat Schroven, Marleen Voet, Rob Lavigne, Hanne Hendrix
      Pages 113-128

  4. Decorating Phages by Conjugation

    1. Front Matter

      Pages 129-129
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    2. Preparation of Bioconjugates of Chimeric M13 Phage and Gold Nanorods

      • Huan Peng, Irene A. Chen
      Pages 131-141

    3. Liquid Glycan Array

      • Chih-Lan Lin, Eric J. Carpenter, Taoran Li, Tareq Ahmed, Ratmir Derda
      Pages 143-159

  6. Probing Phage Biology

    1. Front Matter

      Pages 205-205
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    2. Obtaining Detailed Phage Transcriptomes Using ONT-Cappable-Seq

      • Leena Putzeys, Danish Intizar, Rob Lavigne, Maarten Boon
      Pages 207-235
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Editors and Affiliations

  • Cellular Signaling Laboratory International Research, Center for Sensory Biology and Technology of MOST, Key Laboratory of Molecular Biophysics of MOE, College of Life Science and Technology, Huazhong University of Science and Technology (HUST), Wuhan, China

    Huan Peng

  • College of Life Science and Technology, Huazhong University of Science and Technology (HUST), Wuhan, China

    Jianfeng Liu

  • Department of Chemical and Biomolecular Engineering, Department of Chemistry and Biochemistry, University of California, Los Angeles, USA

    Irene A. Chen

Bibliographic Information

  • Book Title: Phage Engineering and Analysis

  • Book Subtitle: Methods and Protocols

  • Editors: Huan Peng, Jianfeng Liu, Irene A. Chen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3798-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024

  • Hardcover ISBN: 978-1-0716-3797-5Published: 26 March 2024

  • Softcover ISBN: 978-1-0716-3800-2Due: 26 April 2024

  • eBook ISBN: 978-1-0716-3798-2Published: 25 March 2024

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 300

  • Number of Illustrations: 8 b/w illustrations, 45 illustrations in colour

  • Topics: Biotechnology, Biological Techniques, Biochemical Engineering

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