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Flux Balance Analysis of Mammalian Cell Systems

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Mammalian Synthetic Systems

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2774))

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Abstract

Flux balance analysis (FBA) is a computational methodology to model and analyze the metabolic behavior of cells. In this chapter, we break down the key steps for formulating an FBA model and other FBA-derived methodologies in the context of mammalian cell biology, including strain design, developing cell line-specific models, and conducting flux sampling. We provide annotated COBRApy code for each step to show how it would work in practice.

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Acknowledgments

Benjamin Strain would like to thank the UK Biotechnology and Biological Sciences Research Council (BBSRC) and GlaxoSmithKline for their funding and support. James Morrissey thanks the BBSRC and AstraZeneca for their funding and support.

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Authors and Affiliations

  1. Department of Chemical Engineering, Imperial College London, London, UK

    James Morrissey, Benjamin Strain & Cleo Kontoravdi

Authors
  1. James Morrissey
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  2. Benjamin Strain
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  3. Cleo Kontoravdi
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Corresponding author

Correspondence to Cleo Kontoravdi .

Editor information

Editors and Affiliations

  1. Department of Chemical Engineering and Imperial College Centre for Synthetic Biology, Imperial College London, London, UK

    Francesca Ceroni

  2. Department of Chemical Engineering and Imperial College Centre for Synthetic Biology, Imperial College London, London, UK

    Karen Polizzi

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Morrissey, J., Strain, B., Kontoravdi, C. (2024). Flux Balance Analysis of Mammalian Cell Systems. In: Ceroni, F., Polizzi, K. (eds) Mammalian Synthetic Systems. Methods in Molecular Biology, vol 2774. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3718-0_9

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  • DOI: https://doi.org/10.1007/978-1-0716-3718-0_9

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3717-3

  • Online ISBN: 978-1-0716-3718-0

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