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Proliferation of Axillary Shoots of Chestnut in Temporary Immersion Systems

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Micropropagation Methods in Temporary Immersion Systems

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2759))

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Abstract

Shoots from European chestnut and hybrids of European and Asian chestnuts can be efficiently proliferated in liquid medium by temporary immersion (TIS) using RITA® and Plantform™ bioreactors. The main challenges of applying TIS to these species were the lack of growth and hyperhydricity; problem solutions included the manipulation of the hormone concentration, the explant type, immersion frequency, and a support for maintaining shoots in a vertical position. After protocol optimization, explants cultured by TIS produced more rootable shoots than explants growing in semisolid medium, enabling increased number of rooted and acclimatable shoots. In this chapter, we will describe the protocols for proliferating chestnut by TIS in RITA® and Plantform™ bioreactors, together with tips for avoiding the main pitfalls of the technique. The strategies applied to chestnut can be useful for culturing other woody plants in bioreactors.

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Acknowledgments

This research was partially funded by Xunta de Galicia (Spain) through projects 09MRU016E and IN607A 2021/06 and by FEDER INNTERCONECTA 2013/2014 program (Project INTEGRACASTANEA EXP00064828/ITC-20133040). The authors thank Blandina Blanco, Begoña Correa, Anxela Aldrey, Maite García and Begoña Pato for technical assistance and Bruce Christie for critical reading of the manuscript.

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Authors and Affiliations

  1. Department of Plant Production, Misión Biológica de Galicia, Sede Santiago de Compostela, CSIC, Santiago de Compostela, Spain

    Nieves Vidal & Conchi Sánchez

  2. Maceda Nursery, Grupo Tragsa, Maceda, Ourense, Spain

    Beatriz Cuenca

Authors
  1. Nieves Vidal
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  2. Conchi Sánchez
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  3. Beatriz Cuenca
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Corresponding author

Correspondence to Nieves Vidal .

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Editors and Affiliations

  1. Unidad Guerrero Negro, Centro de Investigaciones Biológicas del Noroeste S.C. (CIBNOR), Guerrero Negro, Baja California Sur, Mexico

    Marco A. Ramírez-Mosqueda

  2. Facultad de Ciencias Químicas, Universidad Veracruzana, Orizaba, Veracruz, Mexico

    Carlos A. Cruz-Cruz

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Vidal, N., Sánchez, C., Cuenca, B. (2024). Proliferation of Axillary Shoots of Chestnut in Temporary Immersion Systems. In: Ramírez-Mosqueda, M.A., Cruz-Cruz, C.A. (eds) Micropropagation Methods in Temporary Immersion Systems. Methods in Molecular Biology, vol 2759. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3654-1_16

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  • DOI: https://doi.org/10.1007/978-1-0716-3654-1_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3653-4

  • Online ISBN: 978-1-0716-3654-1

  • eBook Packages: Springer Protocols

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