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Single Cell Analysis

Methods and Protocols

  • Book
  • © 2024

Overview

Editors:
  1. Miodrag Gužvić

    1. Department of Urology, University Hospital Regensburg, Regensburg, Germany

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2752)

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About this book

This volume explores the latest advancements and techniques used to study cell analysis, their capabilities, and the type of results that can be obtained. The chapters in this book cover topics such as FACS; fluorescence microscopy; organic spectroscopy such as MALDI; inorganic spectroscopy such as ICP-MS; and sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Cutting-edge and practical, Single Cell Analysis: Methods and Protocols is a valuable tool for any researcher interested in learning more about this important and developing field. 


Keywords

Table of contents (16 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Single Cell Isolation from Surgically Resected Tissue Via Mechanical Dissociation Using TissueGrinder

    • Prama Pallavi, Stefan Scheuermann
    Pages 1-9

  3. Circulating Tumor Cell Enrichment and Single-Cell Isolation Combining the CellSearch® and DEPArray™ Systems

    • Cäcilia Köstler, Bernhard Polzer, Barbara Alberter
    Pages 11-42

  4. Isolation of Viable Epithelial and Mesenchymal Circulating Tumor Cells from Breast Cancer Patients

    • Justyna Topa, Anna J. Żaczek, Aleksandra Markiewicz
    Pages 43-52

  5. Single-Cell Recovery from Tumor Cell Xenotransplanted Zebrafish Embryos for the Study of Metastasis-Initiating Cells

    • Pablo Hurtado, Inés Martínez-Pena, Roberto Piñeiro
    Pages 53-63

  6. Isolation of Single Circulating Tumor Cells Using VyCAP Puncher System

    • Thais Pereira-Veiga, Bianca Behrens, Joska J. Broekmaat, Lisa Oomens, Michiel Stevens, Arjan G. J. Tibbe et al.
    Pages 65-70

  7. Simultaneous Isolation and Amplification of mRNA and Genomic DNA of a Single Cell

    • Miodrag Gužvić
    Pages 71-100

  8. Isolation and Genomic Analysis of Circulating Tumor Cell Clusters in Cancer Patients

    • Carolina Reduzzi, Marta Vismara, Thomas Schamberger, Marco Silvestri, Rosita Motta, Bernhard M. Polzer et al.
    Pages 101-117

  9. Establishing Single-Cell Clones from In Vitro-Cultured Circulating Tumor Cells

    • Teng Teng, Min Yu
    Pages 119-126

  10. Immunofluorescence Combined with Single-Molecule RNA Fluorescence In Situ Hybridization for Concurrent Detection of Proteins and Transcripts in Stress Granules

    • Jakub Kochan, Mateusz Wawro
    Pages 127-141

  11. Highly Multiplexed and Simultaneous Characterization of Protein and RNA in Single Cells by Flow or Mass Cytometry Platforms Using Proximity Ligation Assay for RNA

    • Andrew D. Duckworth, Joseph R. Slupsky, Nagesh Kalakonda
    Pages 143-165

  12. Array-Based Comparative Genomic Hybridization for the Detection of Copy Number Alterations in Single Cells

    • Giancarlo Feliciello, Zbigniew Tadeusz Czyz, Bernhard M. Polzer
    Pages 167-187

  13. Single Cell Micro RNA Sequencing Library Preparation

    • Sarah M. Hücker, Stefan Kirsch
    Pages 189-199

  14. Immunoblot Analysis from Single Cells Using Milo™ Single-Cell Western Platform

    • Prashant V. Thakkar
    Pages 201-214

  15. Imaging of Subcellular Distribution of Platinum in Single Cells Using Laser Ablation Inductively Coupled Plasma Mass Spectrometry

    • Amy J. Managh, Calum J. Greenhalgh
    Pages 215-226

  16. Patch-seq: Multimodal Profiling of Single-Cell Morphology, Electrophysiology, and Gene Expression

    • Cathryn R. Cadwell, Andreas S. Tolias
    Pages 227-243

  17. Single-Nucleus ATAC-seq for Mapping Chromatin Accessibility in Individual Cells of Murine Hearts

    • Michail Yekelchyk, Xiang Li, Stefan Guenther, Thomas Braun
    Pages 245-257

  18. Back Matter

    Pages 259-260
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Editors and Affiliations

  • Department of Urology, University Hospital Regensburg, Regensburg, Germany

    Miodrag Gužvić

Bibliographic Information

  • Book Title: Single Cell Analysis

  • Book Subtitle: Methods and Protocols

  • Editors: Miodrag Gužvić

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3621-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2024

  • Hardcover ISBN: 978-1-0716-3620-6Published: 10 January 2024

  • Softcover ISBN: 978-1-0716-3623-7Due: 10 February 2024

  • eBook ISBN: 978-1-0716-3621-3Published: 09 January 2024

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 260

  • Number of Illustrations: 9 b/w illustrations, 45 illustrations in colour

  • Topics: Cell Biology, Analytical Chemistry, Materials Science, general

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