Abstract
Primary cells form the basis of modern-day in vitro research analysis tools. Many conventional procedures for generating single-cell suspensions from solid tissue are neither robust nor reproducible. Here we describe primary cells isolation from surgically resected tumor tissue via enzyme-free mechanical dissociation using TissueGrinder, a novel semi-automated benchtop device. The isolated cells can be used for any downstream biochemical or cell-based analytic assay.
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Acknowledgement
This research work was supported by Wilhelm Müller-Stiftung.
Ethical Statement
This study was approved by the local ethics committee of the University Medical Center Mannheim (2012-293 N-MA), and all of the donors gave their written informed consent.
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Pallavi, P., Scheuermann, S. (2024). Single Cell Isolation from Surgically Resected Tissue Via Mechanical Dissociation Using TissueGrinder. In: Gužvić, M. (eds) Single Cell Analysis. Methods in Molecular Biology, vol 2752. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3621-3_1
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DOI: https://doi.org/10.1007/978-1-0716-3621-3_1
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