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Storage and Lyophilization of Pure Proteins

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Protein Chromatography

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2699))

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Abstract

This chapter outlines empirical procedures for the storage of pure proteins with preservation of high levels of biological activity. It describes simple and workable means of preventing microbial contamination and proteolytic degradation and the use of various types of stabilizing additives. It sets out the principles of lyophilization (a complex process comprising freezing, primary drying, and secondary drying stages, otherwise known as freeze-drying). There follows a general procedure for the use of lyophilizer apparatus with emphasis on best practice and on pitfalls to avoid. The use of modulated differential scanning calorimetry to measure the glass transition temperature, a key parameter in the design and successful operation of lyophilization processes, is described. This chapter concludes with brief summaries of interesting recent work in the field.

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Authors and Affiliations

  1. School of Biotechnology, Dublin City University, Dublin, Ireland

    Ciarán Ó’Fágáin

  2. Pfizer Ireland Pharmaceuticals, Grange Castle Business Park, Dublin, Ireland

    Keith Colliton

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  1. Ciarán Ó’Fágáin
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  2. Keith Colliton
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Correspondence to Ciarán Ó’Fágáin .

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Editors and Affiliations

  1. Department of Life and Health Sciences, Dundalk Institute of Technology, Dundalk, Louth, Ireland

    Sinéad T Loughran

  2. National Institute for Bioprocessing Research and Training (NIBRT), Blackrock, Dublin, Ireland

    John Joseph Milne

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Ó’Fágáin, C., Colliton, K. (2023). Storage and Lyophilization of Pure Proteins. In: Loughran, S.T., Milne, J.J. (eds) Protein Chromatography. Methods in Molecular Biology, vol 2699. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3362-5_19

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  • DOI: https://doi.org/10.1007/978-1-0716-3362-5_19

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