Abstract
Human metabolic liver disease is dramatically increasing globally and presents an urgent clinical unmet need. Rodent models of non-alcoholic fatty liver disease (NAFLD) are available, but they fail to fully recreate the metabolic and cellular features of human disease. Thus, it is imperative to understand the metabolic interplay in human cells in the context of disease. We have applied nuclear magnetic resonance (NMR) spectroscopy approaches to enable the detection of numerous metabolites in human cells and within intact tissue in a single measurement. In this chapter, we describe the challenges of using isolated human hepatocytes vs perfused human liver tissue for metabolic tracer experiments and how experimental parameters can be refined to interrogate signals from intact tissue and cells.
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Acknowledgments
This study includes independent research supported by the Birmingham National Institute for Health and Care Research (NIHR) Birmingham Biomedical Research Centre, based at the University of Birmingham. The views expressed are those of the authors and not necessarily those of the NHS, the National Institute for Health and Care Research, or the Department of Health and Social Care. We are grateful to the physicians and patients at the Queen Elizabeth Hospital in Birmingham who donated their tissue for our investigations.
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Saborano, R., Shepherd, E., Günther, U.L., Lalor, P.F. (2023). Tracer-Based Metabolic Analysis by NMR in Intact Perfused Human Liver Tissue. In: Papa, S., Bubici, C. (eds) Metabolic Reprogramming. Methods in Molecular Biology, vol 2675. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3247-5_13
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DOI: https://doi.org/10.1007/978-1-0716-3247-5_13
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