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The Assembly-Line Enzymology of Nonribosomal Peptide Biosynthesis

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Non-Ribosomal Peptide Biosynthesis and Engineering

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2670))

Abstract

Peptide natural products constitute a major class of secondary metabolites produced by microorganisms (mostly bacteria and fungi). In the past several decades, researchers have gained extensive knowledge about nonribosomal peptides (NRPs) generated by ribosome-independent systems, namely, NRP synthetases (NRPSs). NRPSs are multifunctional enzymes consisting of semiautonomous domains that form a peptide backbone. Using a thiotemplate mechanism that employs assembly-line logic with multiple modules, NRPSs activate, tether, and modify amino acid building blocks, sequentially elongating the peptide chain before releasing the complete peptide. Adenylation, thiolation, condensation, and thioesterase domains play central roles in these reactions. This chapter focuses on the current understanding of these central domains in NRPS assembly-line enzymology.

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Authors and Affiliations

  1. Graduate School of Bioscience and Biotechnology, Fukui Prefectural University, Fukui, Japan

    Chitose Maruyama & Yoshimitsu Hamano

  2. Fukui Bioincubation Center (FBIC), Fukui Prefectural University, Fukui, Japan

    Chitose Maruyama & Yoshimitsu Hamano

Authors
  1. Chitose Maruyama
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  2. Yoshimitsu Hamano
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Correspondence to Yoshimitsu Hamano .

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Editors and Affiliations

  1. Department of Chemistry and Biochemistry, University of California San Diego, La Jolla, CA, USA

    Michael Burkart

  2. Faculty of Pharmacy, Kindai University, Higashi-Osaka, Osaka, Japan

    Fumihiro Ishikawa

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Maruyama, C., Hamano, Y. (2023). The Assembly-Line Enzymology of Nonribosomal Peptide Biosynthesis. In: Burkart, M., Ishikawa, F. (eds) Non-Ribosomal Peptide Biosynthesis and Engineering. Methods in Molecular Biology, vol 2670. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3214-7_1

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  • DOI: https://doi.org/10.1007/978-1-0716-3214-7_1

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3213-0

  • Online ISBN: 978-1-0716-3214-7

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