Abstract
Measuring the mitochondrial respiratory capacity of brown adipocytes ex vivo is an essential approach to understand the cell-autonomous regulators of mitochondrial uncoupling in brown adipose tissue. Here, we describe two protocols to isolate brown preadipocytes from mice, their ex vivo differentiation to mature brown adipocytes and the quantification of their mitochondrial uncoupling capacity by respirometry.
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References
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Veliova M, Ferreira CM, Benador IY et al (2020) Blocking mitochondrial pyruvate import in brown adipocytes induces energy wasting via lipid cycling. EMBO Rep 21:e49634
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Acknowledgments
We thank Barbara Cannon, Jan Nedergaard, Jakob Wikstrom, Kiana Mahdaviani, Ilan Benador, and Marcus Fernandes de Oliveira for their mentoring on the protocol using 3–4-week-old mice.
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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Veliova, M., Tol, M.J., Tontonoz, P., Shirihai, O.S., Liesa, M. (2023). Measuring Mitochondrial Uncoupling in Mouse Primary Brown Adipocytes Differentiated Ex Vivo. In: Lodhi, I.J. (eds) Thermogenic Fat. Methods in Molecular Biology, vol 2662. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3167-6_5
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DOI: https://doi.org/10.1007/978-1-0716-3167-6_5
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3166-9
Online ISBN: 978-1-0716-3167-6
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