Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2662)
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Table of contents (22 protocols)
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Front Matter
Keywords
About this book
This volume presents a broad collection of state-of-the-art methods to study the biology of thermogenic fat using in vitro cell culture and animal models. Chapters guide the readers through protocols on differentiation of human pluripotent stem cells and murine adipocyte precursors; methods for measuring mitochondrial respiration, heat generation, brown fat activation, and effects on energy metabolism in mice; and techniques for AAV-mediated gene delivery, transplantation of adipose tissue, isolation of adipose tissue immune cells and extracellular vesicles, and mass spectrometry-based profiling of brown fat lipids. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge,Thermogenic Fat: Methods and Protocols aims to be comprehensive guide for researchers in the field.
Editors and Affiliations
Bibliographic Information
Book Title: Thermogenic Fat
Book Subtitle: Methods and Protocols
Editors: Irfan J. Lodhi
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-3167-6
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023
Hardcover ISBN: 978-1-0716-3166-9Published: 20 April 2023
Softcover ISBN: 978-1-0716-3169-0Published: 21 April 2024
eBook ISBN: 978-1-0716-3167-6Published: 19 April 2023
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 253
Number of Illustrations: 1 b/w illustrations
Topics: Biomedicine, general, Physiology, Stem Cells