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Unraveling Plant-Pathogen Interactions in Cereals Using RNA-seq

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Plant-Pathogen Interactions

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2659))

Abstract

Over the past two decades, there have been significant advancements in the realm of transcriptomics, or the study of genes and their expression. Modern RNA sequencing technologies and high-performance computing are creating a “big data” revolution that provides new opportunities to explore the interactions between cereals and pathogens that affect grain yield and food safety. These data are being used to annotate genes and gene variants, as well as identify differentially expressed genes and create global gene co-expression networks. Moreover, these data can unravel the complex interactions between pathogen and host and identify genes and pathways involved in these interactions. This information can then be used for disease mitigation and the development of crops with superior resistance.

Bronwyn E. Rowland is the primary author to this chapter.

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Author information

Authors and Affiliations

  1. Department of Biomedical and Molecular Sciences, Queen’s University, Kingston, ON, Canada

    Bronwyn E. Rowland

  2. Morden Research and Development Centre, Agriculture and Agri-Food Canada, Morden, MB, Canada

    Maria Antonia Henriquez

  3. Brandon Research and Development Centre, Agriculture and Agri-Food Canada, Brandon, MB, Canada

    Kirby T. Nilsen

  4. Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, Ottawa, ON, Canada

    Rajagopal Subramaniam

  5. Grain Research Laboratory, Canadian Grain Commission, Winnipeg, MB, Canada

    Sean Walkowiak

Authors
  1. Bronwyn E. Rowland
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  2. Maria Antonia Henriquez
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  3. Kirby T. Nilsen
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  4. Rajagopal Subramaniam
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  5. Sean Walkowiak
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Corresponding authors

Correspondence to Kirby T. Nilsen , Rajagopal Subramaniam or Sean Walkowiak .

Editor information

Editors and Affiliations

  1. Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada, Lethbridge, AB, Canada

    Nora A. Foroud

  2. Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada, Lethbridge, AB, Canada

    Jonathan A. D. Neilson

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© 2023 His Majesty the King in Right of Canada, as represented by the Minister of Agriculture and Agri-Food

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Rowland, B.E., Henriquez, M.A., Nilsen, K.T., Subramaniam, R., Walkowiak, S. (2023). Unraveling Plant-Pathogen Interactions in Cereals Using RNA-seq. In: Foroud, N.A., Neilson, J.A.D. (eds) Plant-Pathogen Interactions. Methods in Molecular Biology, vol 2659. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3159-1_9

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  • DOI: https://doi.org/10.1007/978-1-0716-3159-1_9

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3158-4

  • Online ISBN: 978-1-0716-3159-1

  • eBook Packages: Springer Protocols

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