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Generation of Radioresistant Prostate Cancer Cells

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Cancer Cell Culture

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2645))

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Abstract

The development of in vitro isogenic models of radioresistance through exposure to fractionated radiation is an increasingly used approach to investigate the mechanisms of radioresistance in cancer cells. Owing to the complex nature of the biological effect of ionizing radiation, the generation and validation of these models requires the careful consideration of radiation exposure protocols and cellular endpoints. This chapter presents a protocol we used to derive and characterize an isogenic model of radioresistant prostate cancer cells. This protocol may be applicable to other cancer cell lines.

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Author information

Authors and Affiliations

  1. Translational Radiobiology and Oncology Group, Applied Radiation Therapy Trinity Research Group, Trinity College Dublin, Dublin, Ireland

    Laure Marignol

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  1. Laure Marignol
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Correspondence to Laure Marignol .

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Editors and Affiliations

  1. Trinity Translational Medicine Institute, Trinity Centre for Health Sciences, Dublin, Ireland

    Dania Movia

  2. Trinity Translational Medicine Institute, Trinity Centre for Health Sciences, Dublin, Ireland

    Adriele Prina-Mello

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Marignol, L. (2023). Generation of Radioresistant Prostate Cancer Cells. In: Movia, D., Prina-Mello, A. (eds) Cancer Cell Culture. Methods in Molecular Biology, vol 2645. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3056-3_5

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  • DOI: https://doi.org/10.1007/978-1-0716-3056-3_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3055-6

  • Online ISBN: 978-1-0716-3056-3

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