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Methods in Cell Separations

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Cell Separation

Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 106))

Abstract

Research in the field of cell biology and biomedicine relies on technologies that fractionate cell populations and isolate rare cell types to high purity. A brief overview of methods and commercially available products currently used in cell separations is presented. Cell fractionation by size and density and highly selective affinity-based technologies such as affinity chromatography, fluorescence-activated cell sorting (FACS) and magnetic cell sorting are discussed in terms of throughput, yield, and purity.

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References

  1. Geifman-Holtzman O, Makhlouf F, Kaufman L, Gonchoroff NJ, Hlotzman EJ (2000) Am J Obstet Gynecol 183:462

    Article  CAS  Google Scholar 

  2. De Wynter EW, Coutinho LH, Pei X, Marsh JCW, Hows J, Luft T, Testa NG (1995) Stem Cells 13:524

    Article  Google Scholar 

  3. Beaujean F (1997) Transfus Sci 18:251

    Article  CAS  Google Scholar 

  4. Siewert C, Herber M, Hunzelmann N, Fodstad O, Miltenyi S, Assenmacher M, Schmitz J (2001) Recent Results Cancer Res 158:51

    CAS  Google Scholar 

  5. Racila E, Euhus D, Weiss AJ, Rao C, McConnell J, Terstappen L, Uhr J (1998) Proc Natl Acad Sci USA 95:4589

    Article  CAS  Google Scholar 

  6. Prestvik WS, Berge A, Mork PC, Stenstad PM, Ugelstad J (1997) Preparation and application of monosized magnetic particles in selective cell separation. In: Häfeli U, Schutt W, Teller J, Zborowski M (eds) Scientific and clinical applications of magnetic carriers. Plenum, New York, London

    Google Scholar 

  7. Collins DP, Luebering BJ, Shaut DM (1998) Cytometry 33:249

    Article  CAS  Google Scholar 

  8. Peters C, Steward CG (2003) Bone Marrow Transplant 31:229

    Article  CAS  Google Scholar 

  9. Bhattacharya A, Slatter MA, Chapman CE, Barge D, Jackson A, Flood TJ, Abinun M, Cant AJ, Gennery AR (2005) Bone Marrow Transplant 36:295

    Article  CAS  Google Scholar 

  10. Chan J, Siatskas C, Field J, Toh B-H, Alderuccio F (2006) Drug Discov Today: Disease Mechanisms 3:219

    Article  Google Scholar 

  11. Islam D, Lindberg AA (1992) J Clin Microbiol 30:2801

    CAS  Google Scholar 

  12. Payne MJ, Campbell S, Patchett RA, Kroll RG (1992) J Appl Bacteriol 73:41

    CAS  Google Scholar 

  13. Deng MQ, Cliver DO, Mariam TW (1997) Appl Environ Microbiol 63:3134

    CAS  Google Scholar 

  14. Seesod N, Nopparat P, Hedrum A, Holder A, Thaithong S, Uhlen M, Lundeberg J (1997) Am J Trop Med Hyg 56:322

    CAS  Google Scholar 

  15. Shizuru JA, Negrin RS, Weissman IL (2005) Annu Rev Med 56:509

    Article  CAS  Google Scholar 

  16. Kodituwakku AP, Jessup C, Zola H, Roberton AM (2003) Immunol Cell Biol 81:163

    Article  CAS  Google Scholar 

  17. Gross HJ, Verwer B, Houck D, Hoffman RA, Recktenwald D (1995) Proc Natl Acad Sci USA 92:537

    Article  CAS  Google Scholar 

  18. Oshiba A, Renz H, Yata J, Gelfand EW (1994) Clin Immunol Immunopathol 72:342

    Article  CAS  Google Scholar 

  19. Vettese-dadey M (1999) The Scientist 13:21

    Google Scholar 

  20. Pretlow TG, Pretlow TP (eds) (1987) Cell separation – methods and selective applications. Academic, New York

    Google Scholar 

  21. Chianea T, Assidjo NE, Cardot PJP (2000) Talanta 51:835

    Article  CAS  Google Scholar 

  22. Pertoft H (2000) J Biochem Biophys Method 44:1

    Article  CAS  Google Scholar 

  23. Wigzell H, Anderson B (1968) J Exp Med 129:23

    Article  Google Scholar 

  24. Truffa-Bachi P, Wofsy L (1970) Proc Natl Acad Sci USA 66:685

    Article  CAS  Google Scholar 

  25. Edelman GM, Rutishauser U, Millette GF (1971) Proc Natl Acad Sci USA 68:2153

    Article  CAS  Google Scholar 

  26. Wildt RMD, Steenbakkers PG, Pennings AH, Hoogen FHVD, Venrooij WJV, Hoet RM (1997) J Immunol Methods 207:61

    Article  Google Scholar 

  27. Herzenberg LA, Parks D, Sahaf B, Perez O, Roederer M, Herzenberg LA (2002) Clin Chem 48:1819

    CAS  Google Scholar 

  28. Givan AL (2004) Methods Mol Biol 263:1

    Google Scholar 

  29. Safarik I, Safarikova M (1999) J Chromatogr B 722:33

    Article  CAS  Google Scholar 

  30. Bonanno G, Perillo A, Rutella S, de Ritis DG, Mariotti A, Marone M, Meoni F, Scambia G, Leone G, Mancuso S, Pierelli L (2004) Transfusion 44:1087

    Article  Google Scholar 

  31. Boyum A (1968) Scand J Clin Lab Invest 21(Suppl.97):77

    CAS  Google Scholar 

  32. Krause DS, Fackler MJ, Civin CI, May WS (1996) Blood 87:1

    CAS  Google Scholar 

  33. Gordon PR, Leimig T, Babarin-Dorner A, Houston J, Holladay M, Mueller I, Geiger T, Handgretinger R (2003) Bone Marrow Transpl 31:17

    Article  CAS  Google Scholar 

  34. Ebener U, Brinkmann A, Zotova V, Niegemann E, Wehner S (2000) Klin Padiatr 212:90

    Article  CAS  Google Scholar 

  35. Yin AH, Miraglia S, Zanjani ED, Almeida-Porada G, Ogawa M, Leary AG, Olweus J, Kearney J, Buck DW (1997) Blood 90:5002

    CAS  Google Scholar 

  36. Koehl U, Zimmermann S, Esser R, Sørensen J, Gruttner HP, Duchscherer M, Seifried E, Klingebiel T, Schwabe D (2002) Bone Marrow Transpl 29:927

    Article  CAS  Google Scholar 

  37. Lang P, Bader P, Schumm M, Feuchtinger T, Einsele H, Fuhrer M, Weinstock C, Handgretinger R, Kuci S, Martin D, Niethammer D, Greil J (2004) Br J Haematol 124:72

    Article  Google Scholar 

  38. Reth M (1992) Annu Rev Immunol 10:97

    Article  CAS  Google Scholar 

  39. Delves PM, Roitt IM (2000) N Engl J Med 343:37

    Article  CAS  Google Scholar 

  40. Berenson RJ, Bensinger WI, Kalamasz D, Martin P (1986) Blood 67:509

    CAS  Google Scholar 

  41. Berenson RJ, Bensinger WI, Kalamasz D (1986) J Immunol Methods 91:11

    Article  CAS  Google Scholar 

  42. Kumar A, Plieva FM, Galaev IY, Mattiasson B (2003) J Immunol Methods 283:185

    Article  CAS  Google Scholar 

  43. Mahe B, Milpied N, Hermouet S, Robillard N, Moreau P, Letortorec S, Rapp MJ, Bataille R, Harousseau JL (1996) Br J Haematol 92:263

    Article  CAS  Google Scholar 

  44. Lemoli RM, Fortuna A, Motta MR, Rizzi S, Giudice V, Nannetti A, Martinelli G, Cavo M, Amabile M, Mangianti S, Fogli M, Conte R, Tura S (1996) Blood 87:1625

    CAS  Google Scholar 

  45. Shpall EJ, Jones RB, Bearman SI, Franklin WA, Archer PG, Curiel T, Bitter M, Claman HN, Stemmer SM, Purdy M (1994) J Clin Oncol 12:28

    CAS  Google Scholar 

  46. Bell GI (1978) Science 200:618

    Article  CAS  Google Scholar 

  47. Haas W, Schrader JW, Szenberg A (1974) Eur J Immunol 4:565

    Article  CAS  Google Scholar 

  48. Mammen M, Choi S-K, Whitesides GM (1998) Angew Chem Int Ed 37:2754

    Article  Google Scholar 

  49. Dainiak MB, Kumar A, Galaev IY, Mattiasson B (2006) Proc Natl Acad Sci USA 103:849

    Article  CAS  Google Scholar 

  50. Galaev IY, Dainiak MB, Plieva FM, Mattiasson B (2007) Langmuir 23:35

    Article  CAS  Google Scholar 

  51. Kumar A, Rodriguez-Caballero A, Plieva FM, Galaev IY, Nandakumar KS, Kamihira M, Holmdahl R, Orfao A, Mattiasson B (2005) J Mol Recog 18:84

    Article  CAS  Google Scholar 

  52. Hentze H-P, Antonietti M (2002) Rev Mol Biotechnol 90:27

    Article  CAS  Google Scholar 

  53. Gustavsson P-E, Larsson P-O (1999) J Chromatogr A 832:29

    Article  CAS  Google Scholar 

  54. Delude RL (2005) Crit Care Med 33:S426

    Article  Google Scholar 

  55. Ibrahim SF, Engh Van den G (2003) Curr Opin Biotechnol 14:5

    Article  CAS  Google Scholar 

  56. McClatchey KD (1994) Clinical laboratory medicine. Williams and Wilkins, Baltimore

    Google Scholar 

  57. Sasaki DT, Tichenor EH, Lopez F, Combs J, Uchida N, Smith CR, Stokdijk W, Vardanega M, Buckle AM, Chen B (1995) J Hematother 4:503

    Article  CAS  Google Scholar 

  58. Ashcroft RG, Lopez PA (2000) J Immunol Method 243:13

    Article  CAS  Google Scholar 

  59. Chalmers JJ, Zborowski M, Sun L, Moore L (1998) Biotechnol Prog 14:141

    Article  CAS  Google Scholar 

  60. Beaujean F (1997) Transfus Sci 18:251

    Article  CAS  Google Scholar 

  61. Kekarainen T, Mannelin S, Laine J, Jaatinen T (2006) BMC Cell Biol 7:30

    Article  CAS  Google Scholar 

  62. Martin-Henao GA, Picon M, Amill B, Querol S, Ferra C, Granena A, Garcia J (2001) Bone Marrow Transpl 27:683

    Article  CAS  Google Scholar 

  63. Negrin RS, Atkinson K, Leemhuis T, Hanania E, Juttner C, Tierney K, Hu WW, Johnston LJ, Shizuru JA, Stockerl-Goldstein KE, Blume KG, Weissman IL, Bower S, Baynes R, Dansey R, Karanes C, Peters W, Klein J (2000) Biol Blood Marrow Transpl 6:262

    Article  CAS  Google Scholar 

  64. Seidl J, Knuechel R, Kunz-Schughart LA (1999) Cytometry 36:102

    Article  CAS  Google Scholar 

  65. Hu X, Bessette PH, Qian J, Meinhart CD, Daugherty PS, Soh HT (2005) Proc Natl Acad Sci USA 102:15757

    Article  CAS  Google Scholar 

  66. Kato S, Yabe H, Yasui M, Kawa K, Yoshida T, Watanabe A, Osugi Y, Horibe K, Kodera Y (2000) Bone Marrow Transpl 26:1281

    Article  CAS  Google Scholar 

  67. Paulus U, Dreger P, Viehmann K, Von Neuhoff N, Schmitz N (1997) Stem Cells 15:297

    Article  CAS  Google Scholar 

  68. Law P, Ishizawa L, Van de Ven C, Burgess J, Hardwick A, Plunkett M, Gee AP, Cairo M (1993) J Hematother 2:247

    Article  CAS  Google Scholar 

  69. Melnik K, Nakamura M, Comella K, Lasky LC, Zborowski M, Chalmers JJ (2001) Biotechnol Prog 17:907

    Article  CAS  Google Scholar 

  70. Belvedere O, Feruglio C, Malangone W, Bonora ML, Donini A, Dorotea L, Tonutti E, Rinaldi C, Pittino M, Baccarani M, Frate GD, Biffoni F, Sala P, Hilbert DM, Degrassi A (1999) Blood Cells Mol Dis 25:140

    Article  Google Scholar 

  71. Schumm M, Lang P, Taylor G, Kuci S, Klingebiel T, Buhring H-J, Geiselhart A, Niethammer D, Handgretinger R (1999) J Hematother 8:209

    Article  CAS  Google Scholar 

  72. Stamm C, Westphal B, Kleine H-D, Petzsch M, Kittner C, Klinge H, Schumichen C, Nienaber CA, Freund M, Steinhoff G (2003) Lancet 361:45

    Article  Google Scholar 

  73. Gazitt Y, Reading CC, Hoffman R, Wickrema A, Vesole DH, Jagannath S, Condino J, Lee B, Barlogie B, Tricot G (1995) Blood 86:381

    CAS  Google Scholar 

  74. Link H, Arseniev L, Bähre O, Kadar JG, Diedrich H, Poliwoda H (1996) Blood 87:4903

    CAS  Google Scholar 

  75. Bensinger WI, Buckner CD, Shannon-Dorcy K, Rowley S, Appelbaum FR, Benyunes M, Clift R, Martin P, Demirer T, Storb R, Lee M, Schiller G (1996) Blood 88:4132

    CAS  Google Scholar 

  76. Berenson RJ, Bensinger WI, Hill RS, Andrews RG, Garcia-Lopez J, Kalamasz DF, Still BJ, Spitzer G, Buckner CD, Bernstein ID, Thomas ED (1991) Blood 77:1717

    CAS  Google Scholar 

  77. Cardoso AA, Watt SM, Batard P, Li ML, Hatzfeld A, Genevier H, Hatzfeld J (1995) Exp Hematol 23:407

    CAS  Google Scholar 

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Correspondence to Bo Mattiasson .

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Ashok Kumar Igor Yu Galaev Bo Mattiasson

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Dainiak, M.B., Kumar, A., Galaev, I.Y., Mattiasson, B. (2007). Methods in Cell Separations. In: Kumar, A., Galaev, I.Y., Mattiasson, B. (eds) Cell Separation. Advances in Biochemical Engineering/Biotechnology, vol 106. Springer, Berlin, Heidelberg. https://doi.org/10.1007/10_2007_069

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