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Genotyping by Multiplexed Sequencing (GMS) Using SNP Markers

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Plant Genotyping

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2638))

Abstract

SNP-based genotyping has become the most effective approach to generate target-specific data for use in genetic studies. In this chapter, we will describe a high-throughput genotyping method that multiplexes hundreds to thousands of SNP markers in a two-step PCR protocol that can be customized to fit the specific needs of a study.

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References

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Acknowledgments

The GMS manuscript was funded by the USDA-ARS.

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Authors and Affiliations

  1. USDA-ARS Wheat Health, Genetics and Quality Research Unit, Pullman, WA, USA

    Travis M. Ruff, Karol Marlowe & Deven R. See

  2. Department of Plant Pathology, Washington State University, Pullman, WA, USA

    Marcus A. Hooker, Yan Liu & Deven R. See

Authors
  1. Travis M. Ruff
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  2. Karol Marlowe
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  3. Marcus A. Hooker
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  4. Yan Liu
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  5. Deven R. See
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Corresponding author

Correspondence to Deven R. See .

Editor information

Editors and Affiliations

  1. College of Science and Engineering, Biological Sciences, Flinders University, Adelaide, SA, Australia

    Yuri Shavrukov

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Ruff, T.M., Marlowe, K., Hooker, M.A., Liu, Y., See, D.R. (2023). Genotyping by Multiplexed Sequencing (GMS) Using SNP Markers. In: Shavrukov, Y. (eds) Plant Genotyping. Methods in Molecular Biology, vol 2638. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3024-2_2

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  • DOI: https://doi.org/10.1007/978-1-0716-3024-2_2

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3023-5

  • Online ISBN: 978-1-0716-3024-2

  • eBook Packages: Springer Protocols

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