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DNA Manipulation and Analysis

  • Book
  • © 2023

Overview

Editors:
  1. Garry Scarlett

    1. Biophysics Laboratories, School of Biological Sciences, University of Portsmouth, Portsmouth, UK

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2633)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Classical Recombinant DNA Cloning

    • Ana Mikić, Arqam Alomari, Darren M. Gowers
    Pages 1-24

  3. A Sequence- and Ligation-Independent Cloning (SLIC) Procedure for the Insertion of Genes into a Plasmid Vector

    • Robert A. Holland
    Pages 25-32

  4. Molecular Cloning Using In Vivo DNA Assembly

    • Sandra Arroyo-Urea, Jake F. Watson, Javier García-Nafría
    Pages 33-44

  5. Assembling Multiple Fragments: The Gibson Assembly

    • Luisana Avilan
    Pages 45-53

  6. TA Cloning Approaches to Cloning DNA with Damaged Ends DNA

    • Charlotte Ayling
    Pages 55-64

  7. PCR-Based Assembly of Gene Sequences by Thermodynamically Balanced Inside-Out (TBIO) Gene Synthesis

    • Timothy J. Ragan, Helen A. Vincent
    Pages 65-79

  8. Random Mutagenesis by PCR

    • F. A. Myers
    Pages 81-86

  9. In Vitro Site Directed Mutagenesis

    • Michael J. McClellan
    Pages 87-95

  10. Xenopus Transgenesis Using the pGateway System

    • Liliya Nazlamova
    Pages 97-109

  11. CRISPR/Cas9 Gene Disruption Studies in F0 Xenopus Tadpoles: Understanding Development and Disease in the Frog

    • Anita Abu-Daya, Annie Godwin
    Pages 111-130

  12. A CRISPR/Cas-Based Method for Precise DNA Integration in Xenopus laevis Oocytes Followed by Intracytoplasmic Sperm Injection (ICSI) Fertilization

    • Sian Angela Martin
    Pages 131-143

  13. A Lambda-Exonuclease SELEX Method for Generating Aptamers to Bacterial Targets

    • Robert Gowland, Darren M. Gowers
    Pages 145-161

  14. Generation of Functional-RNA Arrays by In Vitro Transcription and In Situ RNA Capture for the Detection of RNA-RNA Interactions

    • Helen A. Vincent, Charlotte A. Henderson, Daniela Lopes Cardoso, Anastasia J. Callaghan
    Pages 163-184

  15. Chemical Synthesis of Oligonucelotide Sequences: Phosphoramidite Chemistry

    • John Brazier
    Pages 185-193

  16. Low Throughput Direct Cycle Sequencing of Polymerase Chain Reaction (PCR) Products

    • George D. Zouganelis, Nikolaos Tairis
    Pages 195-211

  17. Nanopore Sequencing for Mixed Samples

    • Angela H. Beckett, Samuel C. Robson
    Pages 213-234

  18. Ethics, Legality, and Safety for Geneticists

    • Simon E. Kolstoe
    Pages 235-245

  19. Back Matter

    Pages 247-249
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Keywords

About this book

This volume details technologies on recombinant DNA and nucleic acid manipulation that underpin much of the biological sciences and instructions on how to conduct them successfully. Chapters guide the reader through the basics of oligonucleotide synthesis and DNA sequencing; recombinant DNA plasmid work; cell-free experiments and the latest developments in CRISPR approaches to genome modification. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
 
Authoritative and cutting-edge, Gene Modification and Nucleic Acid Technologies aims to be the comprehensive guide for life scientists moving into the field of recombinant DNA and nucleic acid manipulation.

Editors and Affiliations

  • Biophysics Laboratories, School of Biological Sciences, University of Portsmouth, Portsmouth, UK

    Garry Scarlett

Bibliographic Information

  • Book Title: DNA Manipulation and Analysis

  • Editors: Garry Scarlett

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3004-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-3003-7Published: 01 March 2023

  • Softcover ISBN: 978-1-0716-3006-8Published: 28 February 2023

  • eBook ISBN: 978-1-0716-3004-4Published: 28 February 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 249

  • Number of Illustrations: 5 b/w illustrations, 44 illustrations in colour

  • Topics: Genetics and Genomics

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