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Dissection, Fixation, and Standard Staining of Adult Drosophila Ovaries

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Drosophila Oogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2626))

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Abstract

Studies of the Drosophila ovary have provided significant insight into the molecular and cellular processes that control cell function, tissue organization, and animal development. To characterize mutants with defects in oogenesis or to observe the distribution of gene products involved in egg production, the ovaries must be carefully extracted and prepared for analysis. This chapter describes the manual dissection of ovaries from adult Drosophila females, followed by standard fixation and staining of the isolated tissue. Specifically, this chapter provides procedures for simple DNA and F-actin staining to assess cell and tissue morphology, as well as immunostaining to localize proteins of interest.

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Acknowledgments

The author thanks Olivier Devergne, Makayla Gomperts, Julia Wittes, and McKenzie Young for their contributions to this protocol.

Author information

Authors and Affiliations

  1. Department of Biology, University of Evansville, Evansville, IN, USA

    Julie A. Merkle

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  1. Julie A. Merkle
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Correspondence to Julie A. Merkle .

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Editors and Affiliations

  1. Department of Anatomy & Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, IA, USA

    Michelle S. Giedt

  2. Department of Anatomy & Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, IA, USA

    Tina L. Tootle

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Merkle, J.A. (2023). Dissection, Fixation, and Standard Staining of Adult Drosophila Ovaries. In: Giedt, M.S., Tootle, T.L. (eds) Drosophila Oogenesis. Methods in Molecular Biology, vol 2626. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2970-3_3

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  • DOI: https://doi.org/10.1007/978-1-0716-2970-3_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2969-7

  • Online ISBN: 978-1-0716-2970-3

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