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Proteomic Identification of Saliva Proteins as Noninvasive Diagnostic Biomarkers

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Difference Gel Electrophoresis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2596))

Abstract

Many biomedically relevant biomarkers are proteins with characteristic biochemical properties and a relatively restricted subcellular distribution. The comparative and mass spectrometry-based proteomic analysis of body fluids can be particularly instrumental for the targeted identification of novel protein biomarkers with pathological relevance. In this respect, new research efforts in biomarker discovery focus on the systematic mapping of the human saliva proteome, as well as the pathobiochemical identification of disease-related modifications or concentration changes in specific saliva proteins. As a product of exocrine secretion, saliva can be considered an ideal source for the biochemical identification of new disease indicators. Importantly, saliva represents a body fluid that is continuously available for diagnostic and prognostic assessments. This chapter gives an overview of saliva proteomics, including a discussion of the usefulness of both liquid chromatography and two-dimensional gel electrophoresis for efficient protein separation in saliva proteomics.

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Authors and Affiliations

  1. Cork University Dental School and Hospital, Wilton, Cork, Ireland

    Eleanor M. O’Sullivan

  2. Department of Biology, Maynooth University, National University of Ireland, Maynooth, Co. Kildare, Ireland

    Paul Dowling & Kay Ohlendieck

  3. Institute of Physiology, University of Bonn, Bonn, Germany

    Dieter Swandulla

Authors
  1. Eleanor M. O’Sullivan
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  2. Paul Dowling
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  3. Dieter Swandulla
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  4. Kay Ohlendieck
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Corresponding author

Correspondence to Kay Ohlendieck .

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Editors and Affiliations

  1. Maynooth University, MAYNOOTH, Ireland

    Kay Ohlendieck

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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O’Sullivan, E.M., Dowling, P., Swandulla, D., Ohlendieck, K. (2023). Proteomic Identification of Saliva Proteins as Noninvasive Diagnostic Biomarkers. In: Ohlendieck, K. (eds) Difference Gel Electrophoresis. Methods in Molecular Biology, vol 2596. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2831-7_12

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  • DOI: https://doi.org/10.1007/978-1-0716-2831-7_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2830-0

  • Online ISBN: 978-1-0716-2831-7

  • eBook Packages: Springer Protocols

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