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Generation of Multispecies Oral Bacteria Biofilm Models

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Oral Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2588))

Abstract

It is well-recognized that oral biofilms that occur in health and disease have a polymicrobial composition, though these are poorly reflected in the literature, with many studies focussing on simple mono-species biofilm model systems. The utility of polymicrobial biofilm model systems is that they more accurately reflect the oral cavity and allow researchers to ask relevant questions in basic science studies, pharmaceutical screening, and investigating inflammatory interactions. Here we describe the detailed methodology of how to sequentially construct and maintain polymicrobial biofilm models pertinent to caries, periodontal disease, and denture stomatitis.

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Author information

Author notes

  1. Authors “Jason L. Brown” and “Mark C. Butcher” have equally contributed to this chapter.

Authors and Affiliations

  1. Oral Sciences Research Group, College of Medical, Veterinary and Life Sciences, Glasgow University, Glasgow, UK

    Jason L. Brown, Mark C. Butcher, Chandra Lekha Ramalingam Veena, Safa Chogule, William Johnston & Gordon Ramage

  2. Glasgow Biofilm Research Network, Glasgow, UK

    Jason L. Brown, Mark C. Butcher, Chandra Lekha Ramalingam Veena, Safa Chogule, William Johnston & Gordon Ramage

Authors
  1. Jason L. Brown
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  2. Mark C. Butcher
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  3. Chandra Lekha Ramalingam Veena
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  4. Safa Chogule
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  5. William Johnston
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  6. Gordon Ramage
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Corresponding author

Correspondence to Gordon Ramage .

Editor information

Editors and Affiliations

  1. School of Dentistry, The University of Queensland, Herston, QLD, Australia

    Gregory J. Seymour

  2. School of Dentistry, The University of Queensland, Herston, QLD, Australia

    Mary P. Cullinan

  3. Faculty of Dentistry, University of Otago, Dunedin, New Zealand

    Nicholas C.K. Heng

  4. Faculty of Dentistry, University of Otago, Dunedin, New Zealand

    Paul R. Cooper

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Brown, J.L., Butcher, M.C., Veena, C.L.R., Chogule, S., Johnston, W., Ramage, G. (2023). Generation of Multispecies Oral Bacteria Biofilm Models. In: Seymour, G.J., Cullinan, M.P., Heng, N.C., Cooper, P.R. (eds) Oral Biology. Methods in Molecular Biology, vol 2588. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2780-8_12

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  • DOI: https://doi.org/10.1007/978-1-0716-2780-8_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2779-2

  • Online ISBN: 978-1-0716-2780-8

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