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Comprehensive and High-Throughput Method for Quantitative Fingerprinting of Phenolic Compounds

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Mass Spectrometry for Metabolomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2571))

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Abstract

Accurate, robust, and wide-coverage analytical tools are needed in polyphenol research to deal with the high physicochemical complexity of the secondary plant metabolome. In this chapter, a novel method based on reversed-phase ultrahigh-performance liquid chromatography coupled with a diode array detector and mass spectrometry is presented, which enables high-throughput, comprehensive, and quantitative fingerprinting of a broad spectrum of phenolic compounds and related metabolites in different food products. The simplicity, low-cost, and excellent analytical performance of this method would facilitate its implementation in food science for quality control and authenticity purposes.

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Author information

Authors and Affiliations

  1. Instituto de Investigación e Innovación Biomédica de Cádiz (INiBICA), Hospital Universitario Puerta del Mar, Universidad de Cádiz, Cádiz, Spain

    Raúl González-Domínguez

  2. Agrifood Laboratory, Faculty of Experimental Sciences, University of Huelva, Huelva, Spain

    Ana Sayago, María Santos-Martín & Ángeles Fernández-Recamales

  3. International Campus of Excellence CeiA3, University of Huelva, Huelva, Spain

    Ana Sayago, María Santos-Martín & Ángeles Fernández-Recamales

Authors
  1. Raúl González-Domínguez
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  2. Ana Sayago
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  3. María Santos-Martín
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  4. Ángeles Fernández-Recamales
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Corresponding authors

Correspondence to Raúl González-Domínguez or Ángeles Fernández-Recamales .

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Editors and Affiliations

  1. Instituto de Investigación e Innovación Biomédica de Cádiz, Hospital Universitario Puerta del Mar, Cádiz, Spain

    Raúl González-Domínguez

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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González-Domínguez, R., Sayago, A., Santos-Martín, M., Fernández-Recamales, Á. (2023). Comprehensive and High-Throughput Method for Quantitative Fingerprinting of Phenolic Compounds. In: González-Domínguez, R. (eds) Mass Spectrometry for Metabolomics. Methods in Molecular Biology, vol 2571. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2699-3_1

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  • DOI: https://doi.org/10.1007/978-1-0716-2699-3_1

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2698-6

  • Online ISBN: 978-1-0716-2699-3

  • eBook Packages: Springer Protocols

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