Skip to main content
SpringerLink
Log in

Plant Secondary Metabolite Determination Through Analytical Chromatographic Techniques: Recent Trends and Advancement

  • Chapter
  • First Online:
Plant Secondary Metabolites

Abstract

The present chapter presents a summary of analytical instruments like high-pressure thin-layer chromatography, high-pressure liquid chromatography, and gas chromatography- mass spectroscopy for their potential benefits to drug discovery through chemical fingerprints for pharmaceutical, food, and biotechnology industries. The methods outlined in the current chapter are quite robust, rapid, economical, consistent, and effective tools for qualitative and quantitative analysis of natural compounds, and identification of markers could be exploited at the industrial scale and thus have beneficial aspects for human welfare and drug discovery.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 119.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 159.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 159.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Abdelkhalek A, Salem MZM, Ali HM, Kordy AM, Salemf AZM, Behiry SI (2020) Antiviral, antifungal, and insecticidal activities of Eucalyptus bark extract: HPLC analysis of polyphenolic compounds. Microb Pathog 147:104383

    Article  CAS  PubMed  Google Scholar 

  • Adejoke OE, Paulinus NS, Omonigho IH (2020) Chemical and phytochemical analyses of extracts from the leaves of Acalypha wilkesiana, “an herbal plant used for the treatment of various skin disorders”. Ann Sci Technol 5(2):40–48

    Article  Google Scholar 

  • Ahmet EMİR, Ceren EMİR, Buket Bozkurt NÜS (2020) GC/MS analysis of alkaloids in Galanthus fosteri baker and determination of its anticholinesterase activity. Turkish J Pharm Sci 17(1):36

    Article  Google Scholar 

  • Ali M, Rathaur B, Nishad U (2020) Phytochemical screening, physicochemical and antioxidant activity, tlc & finger print of HPTLC, Morus Alba ethanol extraction. Int J Innov Sci Res Technol 5(3):1–14

    Google Scholar 

  • Barny LA, Tasca JA, Sanchez HA, Smith CR, Koptur S, Livshultz T, Minbiole KP (2020) Chemotaxonomic investigation of Apocynaceae for retronecine-type pyrrolizidine alkaloids using HPLC-MS/MS. bioRxiv

    Google Scholar 

  • Belscak-Cvitanovic A, Valinger D, Benkovic M, Tusek AJ, Jurina T, Komes D, Kljusuric JG (2018) Integrated approach for bioactive quality evaluation of medicinal plant extracts using HPLC-DAD, spectrophotometric, near infrared spectroscopy and chemometric techniques. Int J Food Prop 20(3):S2463–S2480

    Google Scholar 

  • Benamar H, Tomassini L, Frezza C, Marouf A, Bennaceur M, Nicoletti M (2020) First study on the pyrrolizidine alkaloids of Pardoglossum cheirifolium (L.) E. Barbier & Mathez.: GC-MS analysis of their volatile components in the whole plant. Nat Prod Res:1–6

    Google Scholar 

  • Bhardwaj P, Naryal A, Thakur MS, Aggarwal NK, Saxena S, Chaurasia OP, Kumar R (2020) Comparative antioxidant, antibacterial, and GC-MS analysis of methanol extract’s fractions and isolation of luteolin from leaves of trans-Himalayan Codonopsis clematidea. Ind Crop Prod 144:112046

    Article  CAS  Google Scholar 

  • Bhatia A, Kaur T, Singh B, Arora R, Arora S (2019) Reverse phase HPLC method validation for estimation of polyphenols in medicinal plants and their possible role in reticence of xanthine oxidase activity. Sep Sci Plus 2(7):237–244

    Article  CAS  Google Scholar 

  • Bodalska A, Kowalczyk A, Włodarczyk M, Fecka I (2020) Analysis of polyphenolic composition of a herbal medicinal product-peppermint tincture. Molecules 25(69):1–19

    Google Scholar 

  • Bubueanu C, Pavaloiu R (2016) HPTLC chromatographic polyphenolic fingerprints of plant species from Eastern Europe. Malays J Med Biol Res 3:41–44

    Google Scholar 

  • Çelik SE, Tufan AN, Bekdeşer B, Özyürek M, Güçlü K, Apak R (2017) Identification and determination of phenolics in Lamiaceae species by UPLC-DAD-ESI-MS/MS. J Chromatogr Sci 55(3):291–300

    Article  PubMed  Google Scholar 

  • Conceicao RS, Reis IMA, Cerqueira APM, Perez CJ, Santos Junior MC, Branco A, Ifa DR, Botura MB (2020) Rapid structural characterisation of benzylisoquinoline and aporphine alkaloids from Ocotea spixiana acaricide extract by HPTLC-DESI-MS. Phytochem Anal 2020:1–11

    Google Scholar 

  • Cortes N, Sierra K, Alzate F, Osorioc EH, Osorio E (2017) Alkaloids of amaryllidaceae as inhibitors of cholinesterases (AChEs and BChEs): an integrated bioguided study. Phytochem Anal 29(2):217–227

    Article  PubMed  Google Scholar 

  • Dinakaran SK, Chelle S, Avasarala H (2019) Profiling and determination of phenolic compounds in poly herbal formulations and their comparative evaluation. J Tradit Complement Med 9:319–327

    Article  PubMed  Google Scholar 

  • Ekin HN, Gokbulut A, Aydin ZU, Donmez AA, Orhan IE (2016) Insight into anticholinesterase and antioxidant potential of thirty-four Rosaceae samples and phenolic characterization of the active extracts by HPLC. Ind Crop Prod 91:104–113

    Article  CAS  Google Scholar 

  • Elansary HO, Szopa A, Kubica P, Ekiert H, Klimek-Szczykutowicz M, El-Ansary DO, Mahmoud EA (2020) Polyphenol profile and antimicrobial and cytotoxic activities of natural Mentha × piperita and Mentha longifolia populations in Northern Saudi Arabia. Processes 8(479):1–17. https://doi.org/10.3390/pr8040479

    Article  CAS  Google Scholar 

  • Geethika K, Sunoj Kumar P (2018) Phytochemical screening and high-performance thin-layer chromatography fingerprint profile of three species of Leucas (Lamiaceae). Ancient Sci Life 37:102–107

    Google Scholar 

  • Gonzalez-Gonzalez RM, Barragan-Mendoza L, Peraza-Campos AL, Muniz-Valencia R, Ceballos-Magana SG, Parra-Delgado H (2019) Validation of an HPLC-DAD method for the determination of plant phenolics. Braz J Pharmacogn 29(5):689–693

    Article  CAS  Google Scholar 

  • Gupta P, Patil D, Patil A (2019) Qualitative HPTLC phytochemical profiling of Careya arborea Roxb. bark, leaves and seeds. 3Biotech 9:311

    Google Scholar 

  • Hakim M, Rathod D, Trivedi DA, Panigrahi J, Gantait S, Patel IC (2018) An effective validated method for HPTLC-fingerprinting of alkaloids and glycosides from multiple plant parts of three Terminalia spp. Israel J Plant Sci 65(1–2):109–117

    Article  Google Scholar 

  • Jesioneka A, Poblocka-Olecha L, Zabiegalab B, Bucinskic A, Krauze-Baranowskaa M, Luczkiewicz M (2018) Validated HPTLC method for determination of ledol and alloaromadendrene in the essential oil fractions of Rhododendron tomentosum plants and in vitro cultures and bioautography for their activity screening. J Chromatogr B 1086:63–72

    Article  Google Scholar 

  • Jiao L, Yan C, Zhang K, Xie J, Zhang Y, Wen Z (2018) Comprehensive determination of nine polyphenols in Polygoni Avicularis Herba with a new HPLC–DAD method and their correlation with the antioxidant activities. J Food Meas Charact 12(3):1593–1600

    Article  Google Scholar 

  • Jo BG, Kang KH, Yang MH (2020) Development and validation of a HPLC–PDA method for the simultaneous determination of berberine, palmatine, geniposide, and paeoniflorin in Haedoksamul-tang. Appl Sci 10(16):5482

    Article  CAS  Google Scholar 

  • Kadam NS, Naik AA, Doshi PJ, Nikam TD (2018) High-performance thin-layer chromatography method for simultaneous determination of antipsychotic and medicinally important five β-carboline alkaloids. J Chromatogr Sci 2019:1–11

    Google Scholar 

  • Kaur P, Pandey DK, Gupta RC, Dey A (2019) Simultaneous microwave assisted extraction and HPTLC quantification of mangiferin, amarogentin, and swertiamarin in Swertia species from Western Himalayas. Ind Crops Prod 132:449–459

    Article  CAS  Google Scholar 

  • Köksal E, Tohma H, Kılıç Ö, Alan Y, Aras A, Gülçin I, Bursal E (2017) Assessment of antimicrobial and antioxidant activities of Nepeta trachonitica: analysis of its phenolic compounds using HPLC-MS/MS. Sci Pharm 85(2):24

    Article  PubMed Central  Google Scholar 

  • Lee HW, Hwang IM, Lee HM, Yang JS, Park EJ, Choi JW et al (2020) Validation and determination of quinolizidine alkaloids (QAs) in lupin products by gas chromatography with flame ionization detection (GC-FID). Anal Lett 53(4):606–613

    Article  Google Scholar 

  • Li J, Huang HY, Wang YZ (2018) Optimized determination of phenolic compounds in Dendrobium officinale stems by reverse-phase high performance liquid chromatography. J Liq Chromatogr Relat Technol 41(9):508–516

    Article  CAS  Google Scholar 

  • Marchelak A, Olszewska MA, Owczarek A (2020) Simultaneous quantification of thirty polyphenols in blackthorn flowers and dry extracts prepared thereof: HPLC-PDA method development and validation for quality control. J Pharm Biomed Anal 184:113121

    Article  CAS  PubMed  Google Scholar 

  • Migues VH, David JM, David JP (2020) Determination of polyphenols in Schinus terebinthifolius Raddi bark extracts and chemometric analysis. Anal Methods 12(11):1478–1485

    Article  CAS  Google Scholar 

  • Mir MA, Bashir N, Alfaify A, Oteef MD (2020) GC-MS analysis of Myrtus communis extract and its antibacterial activity against gram-positive bacteria. BMC Complement Med Ther 20(1):1–9

    Article  Google Scholar 

  • Misra A, Kumar B, Shukla P, Srivastava S (2020) Simultaneous HPTLC-UV quantification of colchicine and gloriosine alkaloids in the natural population of Gloriosa superba L., collected from Eastern Ghats of India for the identification of elite chemotypes. J Liq Chromatogr Relat Technol 43(9–10):351–360

    Article  CAS  Google Scholar 

  • Mundengara D, Sulaiman C, Balachandran I, Chandran KPS (2019) Isolation characterization and high-performance thin-layer chromatographic quantification of active xanthonoid mangiferin from Canscora perfoliata. J Planar Chromatogr 32(3):231–235

    Article  Google Scholar 

  • Nair CVJ, Ahamad S, Khan W, Anjum V, Mathur R (2017) Development and validation of high-performance thin-layer chromatography method for simultaneous determination of polyphenolic compounds in medicinal plants. Pharm Res 9:S67–S73

    Google Scholar 

  • Narayanan J, Marimuthu J (2016) HPTLC fingerprint profile (phenolics) of selected Cyathea species from Western Ghats, South India. Chin J Biol 2016:6420371. 1–7

    Article  Google Scholar 

  • Naz R, Roberts TH, Bano A, Nosheen A, Yasmin H, Hassan MN et al (2020) GC-MS analysis, antimicrobial, antioxidant, antilipoxygenase and cytotoxic activities of Jacaranda mimosifolia methanol leaf extracts and fractions. PLoS One 15(7):e0236319

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Nile SH, Park SW (2014) HPTLC analysis, antioxidant and antigout activity of Indian plants. Iran J Pharm Res 13(2):531–539

    PubMed  PubMed Central  Google Scholar 

  • O Elansary H, Szopa A, Kubica P, Ekiert H, Mattar M, Al-Yafrasi MA et al (2019) Polyphenol profile and pharmaceutical potential of Quercus spp. bark extracts. Plants 8(11):486

    Article  PubMed Central  Google Scholar 

  • Pedan V, Weber C, Do T, Fischer N, Reich E, Rohn S (2018) HPTLC fingerprint profile analysis of cocoa proanthocyanidins depending on origin and genotype. Food Chem 267:277–287

    Article  CAS  PubMed  Google Scholar 

  • Perala K, Ciddi V (2018) Simultaneous quantitative determination of nitidine, chelerythrine and sanguinarine using HPTLC from callus extract of Zanthoxylum rhetsa. Am J Anal Chem 9:386–396

    Article  CAS  Google Scholar 

  • Poornima K, Palanisamy CP, Sundaram S, Kanniappan GV (2017) Chromatographic fingerprinting analysis of secondary metabolites present in ethanolic extract of Tabernaemontana divaricata (L.) R. Br. by HPTLC technique. Anal Chem Lett 7(1):20–29

    Article  CAS  Google Scholar 

  • Rathore AS, Sathiyanarayanan L, Mahadik KR (2016) Determination of major polyphenolic components in Euphoria longana Lam. by validated high performance thin-layer chromatography method and direct analysis in real time mass spectrometry. J Chromatogr Sep Tech 7(4):1000330

    Article  Google Scholar 

  • Rivera-Mondragón A, Broeckx G, Bijttebier S, Naessens T, Fransen E, Kiekens F et al (2019) Ultrasound-assisted extraction optimization and validation of an HPLC-DAD method for the quantification of polyphenols in leaf extracts of Cecropia species. Sci Rep 9(1):1–16

    Article  Google Scholar 

  • Rocamora CR, Ramasamy K, Lim SM, Majeed ABA, Agatonovic-Kustrin S (2020) HPTLC based approach for bioassay-guided evaluation of antidiabetic and neuroprotective effects of eight essential oils of the Lamiaceae family plants. J Pharm Biomed Anal 178:112909

    Article  Google Scholar 

  • Satija S, Tambuwala MM, Pabreja K, Bakshi HA, Chellappan DK, Aljabali AA, Nammi S, Thakur GS, Dureja H, Gupta G, Dua K, Mehta M, Garg M (2020) Development of a novel HPTLC fingerprint method for simultaneous estimation of berberine and rutin in medicinal plants and their pharmaceutical preparations followed by its application in antioxidant assay. J Planar Chromatogr 33:313–319

    Article  CAS  Google Scholar 

  • Seal T (2016) Quantitative HPLC analysis of phenolic acids, flavonoids and ascorbic acid in four different solvent extracts of two wild edible leaves, Sonchus arvensis and Oenanthe linearis of north-eastern region in India. J Appl Pharm Sci 6(02):157–166

    Article  CAS  Google Scholar 

  • Senguttuvan J, Subramaniam P (2016) HPTLC fingerprints of various secondary metabolites in the traditional medicinal herb Hypochaeris radicata L. J Bot 2016:5429625. https://doi.org/10.1155/2016/5429625

    Article  CAS  Google Scholar 

  • Smyrska-Wieleba N, Wojtanowski KK, Mroczek T (2017) Comparative HILIC/ESI-QTOF-MS and HPTLC studies of pyrrolizidine alkaloids in flowers of Tussilago farfara and roots of Arnebia euchroma. Phytochem Lett 20:339–349

    Article  CAS  Google Scholar 

  • Sridhar SNC, Sengupta P, Paul AT (2018) Development and validation of a new HPTLC method for quantification of conophylline in Tabernaemontana divaricata samples obtained from different seasons and extraction techniques: insights into variation of pancreatic lipase inhibitory activity. Ind Crops Prod 111:462–470

    Article  Google Scholar 

  • Srinivasan S, Wankhar W, Rathinasamy S, Rajan R (2016) Free radical scavenging potential and HPTLC analysis of Indigofera tinctoria linn (Fabaceae). J Pharm Anal 6:125–131

    Article  PubMed  Google Scholar 

  • Suleiman WB (2020) In vitro estimation of superfluid critical extracts of some plants for their antimicrobial potential, phytochemistry, and GC–MS analyses. Ann Clin Microbiol Antimicrob 19(1):1–12

    Article  Google Scholar 

  • Tewari R, Gupta M, Ahmad F, Rout PK, Misra L, Patwardhan A, Vasudeva R (2017) Extraction, quantification and antioxidant activities of flavonoids, polyphenols and pinitol from wild and cultivated Saraca asoca bark using RP-HPLC-PDA-RI method. Ind Crop Prod 103:73–80

    Article  CAS  Google Scholar 

  • Tohma H, Köksal E, Kılıç Ö, Alan Y, Yılmaz MA, Gülçin I et al (2016) RP-HPLC/MS/MS analysis of the phenolic compounds, antioxidant and antimicrobial activities of Salvia L. species. Antioxidants 5(4):38

    Article  PubMed Central  Google Scholar 

  • Trujillo-Chacón LM, Pastene-Navarrete ER, Bustamante L, Baeza M, Alarcón-Enos JE, Cespedes-Acuña CL (2020) In vitro micropropagation and alkaloids analysis by GC–MS of Chilean Amaryllidaceae plants: Rhodophiala pratensis. Phytochem Anal 31(1):46–56

    Article  PubMed  Google Scholar 

  • Viapiana A, Struck-Lewicka W, Konieczynski P, Wesolowski M, Kaliszan R (2016) An approach based on HPLC-fingerprint and chemometrics to quality consistency evaluation of Matricaria chamomilla L commercial samples. Front Plant Sci 7:1561

    Article  PubMed  PubMed Central  Google Scholar 

  • Wang Y, Li Y, Cang S, Cai Q, Xu H, Wang Y et al (2020) Qualitative and quantitative analysis of pyrrolizidine alkaloids for the entire process quality control from Senecio scandens to Senecio scandens-containing preparations by high performance liquid chromatography-tandem mass spectrometry. J Mass Spectrom 55(10):e4532

    Article  CAS  PubMed  Google Scholar 

  • Wojciechowska M, Gołębiowski M (2020) SPME-GC/MS analysis of volatile compounds contained in the insect larvae of Tenebrio molitor and Leptinotarsa decemlineata before and after using insecticides. Chem Biodivers 17(4):e1900743

    CAS  PubMed  Google Scholar 

  • Zhao W, Liu M, Shen C, Liu H, Zhang Z, Dai W et al (2020) Differentiation, chemical profiles and quality evaluation of five medicinal Stephania species (Menispermaceae) through integrated DNA barcoding, HPLC-QTOF-MS/MS and UHPLC-DAD. Fitoterapia 141:104453

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Forest Products, College Of Forestry, Dr. Yashwant Singh Parmar University Of Horticulture and Forestry, Solan, Himachal Pradesh, India

    Rohit Sharma & Yash Pal Sharma

  2. Department of Plant Sciences, Central University of Himachal Pradesh, Dharamshala, TAB, Shahpur, Kangra, Himachal Pradesh, India

    Ashun Chaudhary

  3. Department of Animal Sciences, Central University of Himachal Pradesh, Dharamshala, TAB, Shahpur, Kangra, Himachal Pradesh, India

    Sunil Kumar

Authors
  1. Rohit Sharma
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ashun Chaudhary
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yash Pal Sharma
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Sunil Kumar
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Rohit Sharma .

Editor information

Editors and Affiliations

  1. Department of Biotechnology, Maharishi Markandeshwar (Deemed to be University), Mullana, Ambala, Haryana, India

    Anil Kumar Sharma

  2. Department of Chemistry, Career Point University, Hamirpur, Himachal Pradesh, India

    Ajay Sharma

Rights and permissions

Reprints and permissions

Copyright information

© 2022 The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd.

About this chapter

Check for updates. Verify currency and authenticity via CrossMark

Cite this chapter

Sharma, R., Chaudhary, A., Sharma, Y.P., Kumar, S. (2022). Plant Secondary Metabolite Determination Through Analytical Chromatographic Techniques: Recent Trends and Advancement. In: Sharma, A.K., Sharma, A. (eds) Plant Secondary Metabolites. Springer, Singapore. https://doi.org/10.1007/978-981-16-4779-6_3

Download citation

Publish with us

Policies and ethics

Search

Navigation