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Isolation and Purification of Chromaffin Granules from Adrenal Glands and Cultured Neuroendocrine Cells

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Chromaffin Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2565))

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Abstract

Chromaffin granules isolated from adrenal glands constitute a powerful experimental tool to the study of secretory vesicle components and their participation in fusion and docking processes, vesicle aggregation, and interactions with cytosolic components. Although it is possible to isolate and purify chromaffin granules from adrenal glands of different species, bovine adrenal glands are the most used tissue source due to its easy handling and the large amount of granules that can be obtained from this tissue. In this chapter, we describe an easy-to-use and short-term protocol for efficiently obtaining highly purified chromaffin granules from bovine adrenal medulla. We additionally include protocols to isolate granules from cultured bovine chromaffin cells and PC12 cells, as well as a section to obtain chromaffin granules from mouse adrenal glands.

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Acknowledgments

We thank Ms. María José Guerra and Daniela M. Ponce for providing images of adrenal glands. This work was supported by ACE210014 from ICM-ANID and FONDECYT 1220825 (to A.M.C.), Chile.

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Authors and Affiliations

  1. Centro Interdisciplinario de Neurociencia de Valparaíso, Facultad de Ciencias, Universidad de Valparaíso, Valparaíso, Chile

    Arlek González-Jamett, María Constanza Maldifassi & Ana María Cárdenas

  2. Escuela de Química y Farmacia, Facultad de Farmacia, Universidad de Valparaíso, Valparaíso, Chile

    Arlek González-Jamett

Authors
  1. Arlek González-Jamett
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  2. María Constanza Maldifassi
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  3. Ana María Cárdenas
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Corresponding author

Correspondence to Ana María Cárdenas .

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Editors and Affiliations

  1. Department of Physical Medicine and Pharmacology, Universidad de La Laguna, La Laguna, Spain

    Ricardo Borges

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González-Jamett, A., Maldifassi, M.C., Cárdenas, A.M. (2023). Isolation and Purification of Chromaffin Granules from Adrenal Glands and Cultured Neuroendocrine Cells. In: Borges, R. (eds) Chromaffin Cells. Methods in Molecular Biology, vol 2565. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2671-9_19

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  • DOI: https://doi.org/10.1007/978-1-0716-2671-9_19

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2670-2

  • Online ISBN: 978-1-0716-2671-9

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