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FISHing for Chromosome Instability and Aneuploidy in the Alzheimer’s Disease Brain

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Alzheimer’s Disease

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2561))

Abstract

Fluorescence in situ hybridization (FISH) is the method of choice for visualizing chromosomal DNA in post-mitotic cells. The availability of chromosome-enumeration (centromeric), site-specific, and multicolor-banding DNA probes offers opportunities to uncover genomic changes, at the chromosomal level, in single interphase nuclei. Alzheimer’s disease (AD) has been associated repeatedly with (sub)chromosome instability and aneuploidy, likely affecting the brain. Although the types and rates of chromosome instability in the AD brain remain a matter of debate, molecular cytogenetic analysis of brain cells appears to be important for uncovering mechanisms of neurodegeneration. Here, we describe a FISH protocol for studying chromosome instability and aneuploidy in the AD brain.

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Acknowledgments

The help of Dr. Oxana S. Kurinnaia is acknowledged. This work was partially supported by RFBR and CITMA according to the research project no. 18–515-34005. Vorsanova’s lab was supported by the Government Assignment of the Russian Ministry of Health, Assignment 121031000238-1. Yurov's lab was supported by the Government Assignment of the Russian Ministry of Science and Higher Education, Assignment AAAA-A19–119040490101-6.

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Authors and Affiliations

  1. Yurov’s Laboratory of Molecular Genetics and Cytogenomics of the Brain, Mental Health Research Center, Moscow, Russia

    Yuri B. Yurov, Svetlana G. Vorsanova & Ivan Y. Iourov

  2. Vorsanova’s Laboratory of Molecular Cytogenetics of Neuropsychiatric Diseases, Veltischev Research and Clinical Institute for Pediatrics of the Pirogov Russian National Research Medical University, Moscow, Russia

    Yuri B. Yurov, Svetlana G. Vorsanova & Ivan Y. Iourov

Authors
  1. Yuri B. Yurov
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  2. Svetlana G. Vorsanova
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  3. Ivan Y. Iourov
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  1. Translational Neuroscience Initiative, Neuroscience Drug Discovery, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA, USA

    Jerold Chun

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Yurov, Y.B., Vorsanova, S.G., Iourov, I.Y. (2023). FISHing for Chromosome Instability and Aneuploidy in the Alzheimer’s Disease Brain. In: Chun, J. (eds) Alzheimer’s Disease. Methods in Molecular Biology, vol 2561. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2655-9_10

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  • DOI: https://doi.org/10.1007/978-1-0716-2655-9_10

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