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Analysis of Peripherally Derived Treg in the Intestine

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Regulatory T-Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2559))

Abstract

Elucidation of the symbiotic relationship between the host and its gut microbiota is critically important for understanding host pathophysiology. Peripherally derived regulatory T cells (pTregs) are recognized as central to immune homeostasis in the intestine. Moreover, the gut microbiota nourishes the intestinal and systemic immune systems, including pTreg, via their metabolites and other components. Therefore, methods to detect pTreg as well as to analyze the interactions between the gut microbiota and pTreg are important for better understanding of the symbiotic relationship with these microorganisms. Here, we describe a protocol to isolate colonic lamina propria cells and analyze pTregs in mice.

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Acknowledgments

We thank Dr. Eiji Miyauchi for helpful comments and Dr. Peter D. Burrows for editing.

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Authors and Affiliations

  1. Laboratory for Intestinal Ecosystem, RIKEN Center for Integrative Medical Sciences, Kanagawa, Japan

    Tadashi Takeuchi & Hiroshi Ohno

Authors
  1. Tadashi Takeuchi
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  2. Hiroshi Ohno
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Corresponding author

Correspondence to Hiroshi Ohno .

Editor information

Editors and Affiliations

  1. Imperial College London, London, UK

    Masahiro Ono

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Takeuchi, T., Ohno, H. (2023). Analysis of Peripherally Derived Treg in the Intestine. In: Ono, M. (eds) Regulatory T-Cells. Methods in Molecular Biology, vol 2559. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2647-4_4

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  • DOI: https://doi.org/10.1007/978-1-0716-2647-4_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2646-7

  • Online ISBN: 978-1-0716-2647-4

  • eBook Packages: Springer Protocols

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