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Human and Rodent Cell Lines as Models of Functional Melatonin-Responsive Pancreatic Islet Cells

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Melatonin

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2550))

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Abstract

Cell culture of different pancreatic islet cell lines, like the murine α-cell line αTC1.9, the rat β-cell lines INS-1 and INS-1 832/13, and the human δ-cell line QGP-1, can serve as valuable cell models for the analysis of melatonin-dependent modulation of hormone secretion. The paper summarizes in detail the requirements of culture for each cell line and includes batch protocols to stimulate hormone secretion and to treat cells with several melatonin concentrations as previously published. We here describe the processing of collected cell pellets or cell culture supernatants as well as different methods to analyze cell experiments after melatonin treatment on the basis of our own experience. Finally, we outlined for each cell line under which conditions the melatonin treatment should be performed to gain reproducible results.

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Acknowledgments

Methods are based on investigations performed by the former research group of Prof. Dr. Elmar Peschke. Melatonin research was supported by the Saxon Academy of Sciences and Humanities in Leipzig. For technical support we thank Franziska Knöfel and Susann Möschter.

Author information

Authors and Affiliations

  1. Institute of Anatomy and Cell Biology, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany

    Juliane Zibolka, Ina Bähr, Elmar Peschke & Ivonne Bazwinsky-Wutschke

  2. Saxon Academy of Sciences and Humanities in Leipzig, Leipzig, Germany

    Eckhard Mühlbauer

Authors
  1. Juliane Zibolka
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  2. Ina Bähr
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  3. Elmar Peschke
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  4. Eckhard Mühlbauer
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  5. Ivonne Bazwinsky-Wutschke
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Corresponding author

Correspondence to Ivonne Bazwinsky-Wutschke .

Editor information

Editors and Affiliations

  1. Inserm, U1016, Institut Cochin, PARIS, France

    Ralf Jockers

  2. Inserm, U1016, Institut Cochin, PARIS, France

    Erika Cecon

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Zibolka, J., Bähr, I., Peschke, E., Mühlbauer, E., Bazwinsky-Wutschke, I. (2022). Human and Rodent Cell Lines as Models of Functional Melatonin-Responsive Pancreatic Islet Cells. In: Jockers, R., Cecon, E. (eds) Melatonin. Methods in Molecular Biology, vol 2550. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2593-4_35

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  • DOI: https://doi.org/10.1007/978-1-0716-2593-4_35

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2592-7

  • Online ISBN: 978-1-0716-2593-4

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